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本文引用的文献

1
GATA factor switching during erythroid differentiation.GATA 因子在红细胞分化过程中的转换。
Curr Opin Hematol. 2010 May;17(3):163-8. doi: 10.1097/MOH.0b013e32833800b8.
2
A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf-Hirschhorn syndrome.一种组蛋白H3赖氨酸36三甲基转移酶将Nkx2-5与沃尔夫-赫希霍恩综合征联系起来。
Nature. 2009 Jul 9;460(7252):287-91. doi: 10.1038/nature08086. Epub 2009 May 31.
3
Mesodermal patterning activity of SCL.SCL的中胚层模式形成活性
Exp Hematol. 2008 Dec;36(12):1593-603. doi: 10.1016/j.exphem.2008.07.005. Epub 2008 Sep 21.
4
A conserved role for Hox paralog group 4 in regulation of hematopoietic progenitors.Hox同源异型基因4旁系同源组在造血祖细胞调控中的保守作用。
Stem Cells Dev. 2009 Jun;18(5):783-92. doi: 10.1089/scd.2008.0227.
5
ER71 acts downstream of BMP, Notch, and Wnt signaling in blood and vessel progenitor specification.ER71在血液和血管祖细胞特化过程中作用于骨形态发生蛋白（BMP）、Notch和Wnt信号通路的下游。
Cell Stem Cell. 2008 May 8;2(5):497-507. doi: 10.1016/j.stem.2008.03.008.
6
Vessel and blood specification override cardiac potential in anterior mesoderm.血管和血液特化优先于前中胚层中的心脏潜能。
Dev Cell. 2007 Aug;13(2):254-67. doi: 10.1016/j.devcel.2007.05.012.
7
Expression of GATA-1 in a non-hematopoietic cell line induces beta-globin locus control region chromatin structure remodeling and an erythroid pattern of gene expression.GATA-1在非造血细胞系中的表达可诱导β-珠蛋白基因座控制区染色质结构重塑及基因表达的红系模式。
J Mol Biol. 2007 Feb 23;366(3):737-44. doi: 10.1016/j.jmb.2006.11.094. Epub 2006 Dec 6.
8
A common progenitor at the heart of development.发育核心处的一个共同祖细胞。
Cell. 2006 Dec 15;127(6):1101-4. doi: 10.1016/j.cell.2006.11.031.
9
Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart.哺乳动物心脏中双潜能心肌和平滑肌细胞前体的发育起源。
Cell. 2006 Dec 15;127(6):1137-50. doi: 10.1016/j.cell.2006.10.028. Epub 2006 Nov 22.
10
Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages.多能性flk-1+心血管祖细胞可分化为心肌细胞、内皮细胞和血管平滑肌谱系。
Dev Cell. 2006 Nov;11(5):723-32. doi: 10.1016/j.devcel.2006.10.002.

Nkx2-5在胚胎发育过程中抑制Gata1基因表达并调节心脏祖细胞的细胞命运。

Nkx2-5 represses Gata1 gene expression and modulates the cellular fate of cardiac progenitors during embryogenesis.

作者信息

Caprioli Arianna, Koyano-Nakagawa Naoko, Iacovino Michelina, Shi Xiaozhong, Ferdous Anwarul, Harvey Richard P, Olson Eric N, Kyba Michael, Garry Daniel J

机构信息

Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, USA.

出版信息

Circulation. 2011 Apr 19;123(15):1633-41. doi: 10.1161/CIRCULATIONAHA.110.008185. Epub 2011 Apr 4.

DOI:10.1161/CIRCULATIONAHA.110.008185
PMID:21464046
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3110259/
Abstract

BACKGROUND

Recent studies suggest that the hematopoietic and cardiac lineages have close ontogenic origins, and that an early mesodermal cell population has the potential to differentiate into both lineages. Studies also suggest that specification of these lineages is inversely regulated. However, the transcriptional networks that govern the cell fate specification of these progenitors are incompletely defined.

METHODS AND RESULTS

Here, we show that Nkx2-5 regulates the hematopoietic/erythroid fate of the mesoderm precursors early during cardiac morphogenesis. Using transgenic technologies to isolate Nkx2-5 expressing cells, we observed an induction of the erythroid molecular program, including Gata1, in the Nkx2-5-null embryos. We further observed that overexpression of Nkx2-5 with an Nkx2-5-inducible embryonic stem cell system significantly repressed Gata1 gene expression and suppressed the hematopoietic/erythroid potential, but not the endothelial potential, of the embryonic stem cells. This suppression was cell-autonomous, and was partially rescued by overexpressing Gata1. In addition, we demonstrated that Nkx2-5 binds to the Gata1 gene enhancer and represses the transcriptional activity of the Gata1 gene.

CONCLUSIONS

Our results demonstrate that the hematopoietic/erythroid cell fate is suppressed via Nkx2-5 during mesodermal fate determination, and that the Gata1 gene is one of the targets that are suppressed by Nkx2-5.

摘要

背景

最近的研究表明，造血谱系和心脏谱系有着密切的个体发生起源，并且早期中胚层细胞群体具有分化为这两个谱系的潜力。研究还表明，这些谱系的特化受到反向调节。然而，控制这些祖细胞命运特化的转录网络尚未完全明确。

方法与结果

在此，我们表明Nkx2-5在心脏形态发生早期调节中胚层前体细胞的造血/红系命运。利用转基因技术分离表达Nkx2-5的细胞，我们在Nkx2-5基因敲除胚胎中观察到红系分子程序的诱导，包括Gata1。我们进一步观察到，使用Nkx2-5诱导型胚胎干细胞系统过表达Nkx2-5可显著抑制Gata1基因表达，并抑制胚胎干细胞的造血/红系潜能，但不影响其内皮潜能。这种抑制是细胞自主性的，并且通过过表达Gata1可部分挽救。此外，我们证明Nkx2-5与Gata1基因增强子结合并抑制Gata1基因的转录活性。

结论

我们的结果表明，在中胚层命运决定过程中，造血/红系细胞命运通过Nkx2-5受到抑制，并且Gata1基因是被Nkx2-5抑制的靶标之一。