Zeighami Habib, Nejad-Dost Gholamreza, Parsadanians Angineh, Daneshamouz Shahrzad, Haghi Fakhri
Department of Microbiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Toxicol Rep. 2019 Dec 23;7:89-92. doi: 10.1016/j.toxrep.2019.12.006. eCollection 2020.
Food safety has emerged as an important global issue with international trade and public health implications. is an important cause of food poisoning worldwide. A total of 200 individual meat samples were collected from meat retail outlets and restaurants and investigated the frequency of and hemolysin BL (Hbl), non-hemolytic enterotoxin (Nhe) complex genes. The meat samples were immediately homogenized and cultured on selective agar and subjected for confirmatory biochemical tests and molecular detection of and n genes. A total of 29 (14.5 %) meat samples were positive for the presence of The frequency of in raw meat (14.1 %) was similar to cooked beef samples (15 %) ( > 0.05). Twenty six (89.6 %) isolates carried at least one or more enterotoxin genes. We found (58.6 %) and (51.7 %) genes with higher frequency than others. Hemolysin BL complex genes were found in lower frequency than Nhe complex ( > 0.05). Detection of enterotoxigenic in meat samples shows a probable risk for public health. Therefore, the reliable molecular methods for monitoring of potentially pathogenic are strongly recommended for the routine food examination.
食品安全已成为一个具有国际贸易和公共卫生影响的重要全球问题。[具体细菌名称未给出]是全球食物中毒的一个重要原因。从肉类零售店和餐馆共采集了200份个体肉类样本，调查了[具体毒素名称未给出]和溶血素BL（Hbl）、非溶血肠毒素（Nhe）复合基因的频率。将肉类样本立即匀浆并在[具体选择性琼脂名称未给出]选择性琼脂上培养，然后进行确证性生化试验以及[具体毒素名称未给出]和n基因的分子检测。共有29份（14.5%）肉类样本检测出[具体毒素名称未给出]呈阳性。生肉中[具体毒素名称未给出]的频率（14.1%）与熟牛肉样本（15%）相似（P＞0.05）。26株（89.6%）分离株携带至少一种或多种肠毒素基因。我们发现[具体毒素基因名称未给出]（58.6%）和[具体毒素基因名称未给出]（51.7%）基因的频率高于其他基因。溶血素BL复合基因的频率低于Nhe复合物（P＞0.05）。肉类样本中肠毒素产生性[具体细菌名称未给出]的检测表明对公众健康存在潜在风险。因此，强烈建议采用可靠的分子方法监测潜在致病性[具体细菌名称未给出]，用于常规食品检测。
