Department of Neurology, Taizhou Hospital, Wenzhou Medical College, Taizhou, Zhejiang, 317000 PR China.
Vasc Med. 2011 Feb;16(1):13-8. doi: 10.1177/1358863X10394237.
The aim of the current study was to explore the possible association of the polymorphism of C(-735)T in MMP-2 with the vulnerable plaque risk in ultrasound-confirmed carotid atherosclerosis cases. Serum MMP-2 levels were measured to investigate the relationship between the MMP-2 level and the genetic variability. The MMP-2 polymorphism was detected by PCR-RFLP in the 243 cases with stable plaque and 221 cases with vulnerable plaque. Serum MMP-2 levels were measured with ELISA. The results showed that MMP-2 was significantly higher in the cases with vulnerable plaque than in the cases with stable plaque. A statistical difference was found between the genotype distributions in the vulnerable plaque cases and that in the stable cases. T-allele frequency was also found to be over-represented in the stable plaque cases than in the vulnerable plaque cases, which might partially explain the observed difference in the serum MMP-2 levels in the different plaque cases. The current results also suggested that MMP-2 was a risk factor in the cases with vulnerable plaques, whereas TT genotype and T allele might be protective factors in the cases with vulnerable plaques.
本研究旨在探讨 MMP-2 基因 C(-735)T 多态性与经超声证实的颈动脉粥样硬化患者易损斑块风险之间的可能关联。测量血清 MMP-2 水平以研究 MMP-2 水平与遗传变异之间的关系。通过 PCR-RFLP 在 243 例稳定斑块病例和 221 例易损斑块病例中检测 MMP-2 多态性。采用 ELISA 法检测血清 MMP-2 水平。结果表明，易损斑块病例的 MMP-2 明显高于稳定斑块病例。易损斑块病例与稳定斑块病例的基因型分布存在统计学差异。T 等位基因频率在稳定斑块病例中也高于易损斑块病例，这可能部分解释了不同斑块病例中血清 MMP-2 水平的差异。本研究结果还提示 MMP-2 是易损斑块病例的危险因素，而 TT 基因型和 T 等位基因可能是易损斑块病例的保护因素。
