Zhu Feng, Jin Xiao-Ping, Zhu Min, Zhang Ling-Ling, Wang Feng, Wang Wan-Fen, Hu Xiao-Fei, Li Wei-Ling, Li Cai, Zheng Zhou
Department of Neurology, Taizhou Hospital, Affiliated Hospital of Wenzhou Medical College Taizhou, Zhejiang Province, P.R. China.
Int J Clin Exp Med. 2013 Aug 1;6(7):567-75. Print 2013.
Matrix metalloproteinase 10 (MMP10) plays an important role in ischemic stroke and has a close relationship with some stroke risk factors. The aim of this study was to investigate the relationship between two single nucleotide polymorphisms (SNP) in the exon regions of the MMP10 gene and atherothrombotic cerebral infarction risk.
Five hundred and thirty-seven hospital-based patients who had suffered first atherothrombotic cerebral infarction and 580 unrelated healthy controls were enrolled. Demographic and clinical features of the subjects were recorded, and two polymorphisms, rs17435959 (G>C), rs17293607 (C>T) were chosen to be genotyped by real-time polymerase chain reaction-restriction TaqMan probes using the ABI 7300 TaqMan platform.
There were several clinical parameters, such as blood pressure, fasting blood glucose, total cholesterol, homocysteine, as well as carotid plaque and smoking, but not average age and sex ratios that showed significant differences between patients and control subjects. For rs17435959, there was no significant difference between the ischemic stroke group and the healthy control group in genotype frequency (OR=1.295, P=0.187, 95% CI (0.882-1.899)) or allele frequency (OR=1.267, P=0.202, 95% CI (0.881-1.823)). Moreover, in smoking, none smoking, having carotid plaque, no carotid plaque, male or female subtypes, there was significant difference between patients and control subjects in genotype frequencies or allele frequencies. The minor allele frequency of rs17293607 was 0.92%, prohibiting further study of this allele.
These findings suggest that the rs17435959 SNP may not associated with atherothrombotic cerebral infarction risk. We also found that rs17293607 is not polymorphic in our study population.
基质金属蛋白酶10（MMP10）在缺血性卒中中起重要作用，且与一些卒中危险因素密切相关。本研究旨在探讨MMP10基因外显子区域的两个单核苷酸多态性（SNP）与动脉粥样硬化性脑梗死风险之间的关系。
纳入537例首次发生动脉粥样硬化性脑梗死的住院患者及580例无亲缘关系的健康对照。记录受试者的人口统计学和临床特征，选择两个多态性位点rs17435959（G>C）、rs17293607（C>T），采用ABI 7300 TaqMan平台，通过实时聚合酶链反应-限制性TaqMan探针进行基因分型。
患者与对照之间在血压、空腹血糖、总胆固醇、同型半胱氨酸以及颈动脉斑块和吸烟等几个临床参数上存在显著差异，但平均年龄和性别比例无显著差异。对于rs17435959，缺血性卒中组与健康对照组在基因型频率（OR=1.295，P=0.187，95%CI（0.882-1.899））或等位基因频率（OR=1.267，P=0.202，95%CI（0.881-1.823））上无显著差异。此外，在吸烟、不吸烟、有颈动脉斑块、无颈动脉斑块、男性或女性亚组中，患者与对照在基因型频率或等位基因频率上存在显著差异。rs17293607的次要等位基因频率为0.92%，无法对该等位基因进行进一步研究。
这些发现表明，rs17435959 SNP可能与动脉粥样硬化性脑梗死风险无关。我们还发现rs17293607在我们的研究人群中不存在多态性。
