Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
ISME J. 2011 Oct;5(10):1595-608. doi: 10.1038/ismej.2011.38. Epub 2011 Apr 7.
Experimental analysis of gut microbial communities and their interactions with vertebrate hosts is conducted predominantly in domesticated animals that have been maintained in laboratory facilities for many generations. These animal models are useful for studying coevolved relationships between host and microbiota only if the microbial communities that occur in animals in lab facilities are representative of those that occur in nature. We performed 16S rRNA gene sequence-based comparisons of gut bacterial communities in zebrafish collected recently from their natural habitat and those reared for generations in lab facilities in different geographic locations. Patterns of gut microbiota structure in domesticated zebrafish varied across different lab facilities in correlation with historical connections between those facilities. However, gut microbiota membership in domesticated and recently caught zebrafish was strikingly similar, with a shared core gut microbiota. The zebrafish intestinal habitat therefore selects for specific bacterial taxa despite radical differences in host provenance and domestication status.
实验分析肠道微生物群落及其与脊椎动物宿主的相互作用主要在经过多代实验室饲养的家养动物中进行。这些动物模型对于研究宿主和微生物群落之间的共同进化关系是有用的，前提是在实验室设施中发生的微生物群落与在自然界中发生的微生物群落具有代表性。我们对最近从其自然栖息地采集的和在不同地理位置的实验室设施中饲养了几代的斑马鱼的肠道细菌群落进行了基于 16S rRNA 基因序列的比较。家养斑马鱼的肠道微生物结构模式在不同的实验室设施中存在差异，与这些设施之间的历史联系有关。然而，家养和最近捕获的斑马鱼的肠道微生物群落成员惊人地相似，具有共享的核心肠道微生物群落。因此，尽管宿主来源和驯化状态存在根本差异，但斑马鱼肠道栖息地仍然选择了特定的细菌分类群。
