Zhang Jing, Yuan Ke, Zhou Wen-Long, Zhou Jian, Yang Ping
The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin'an-311 300, P.R.
Pharmacogn Mag. 2011 Jan;7(25):35-9. doi: 10.4103/0973-1296.75899.
The total flavonoid (TF) and total phenolic (TP) contents of the ethanol extracts of the whole plant, stem, leaf, and seed of Mimosa pudica Linn belonging to the genus Mimosa (Family: Fabaceae alt. Leguminosae), which originates from the subtropical regions of southern China, were determined in this experiment.
The antioxidant activity of the extracts and 5 flavonoid monomers of M. pudica Linn. were also evaluated by 2 assays, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and ferric reducing/antioxidant power (FRAP) assays. In addition, correlation analysis was also made in the present study.
The results showed that leaf extracts contained the highest amount of TF and TP, and the content was significantly higher than that found in other parts of the plant. Moreover, the sequence of antioxidant activity of the ethanol extracts was as follows: leaf > the whole plant > seed > stem; the sequence of the 5 flavonoid monomers was as follows: 5,7,3´,4´-tetrahydroxy-6-C-[β-D-apiose-(1→4)]-β-D-glycopyranosyl flavone (1) > isorientin (2) > orientin (3) > isovitexin (4) > vitexin (5), and the antioxidant activity of compound 1 is equivalent to the synthetic antioxidant trolox or a bit stronger than trolox, and significant correlations were found among the active ingredient contents and the results of antioxidant activity.
The present study suggested that M. pudica Linn. could be a potential rich source of natural antioxidants.
本实验测定了含羞草（含羞草属，豆科）全株、茎、叶和种子乙醇提取物中的总黄酮（TF）和总酚（TP）含量，该植物原产于中国南方亚热带地区。
还通过两种测定方法评估了含羞草提取物和5种黄酮单体的抗氧化活性，即1,1-二苯基-2-苦基肼（DPPH）自由基清除活性测定和铁还原/抗氧化能力（FRAP）测定。此外，本研究还进行了相关性分析。
结果表明，叶提取物中TF和TP含量最高，且显著高于植物其他部位。此外，乙醇提取物的抗氧化活性顺序如下：叶＞全株＞种子＞茎；5种黄酮单体的顺序如下：5,7,3´,4´-四羟基-6-C-[β-D-芹菜糖基-(1→4)]-β-D-吡喃葡萄糖基黄酮（1）＞异荭草素（2）＞荭草素（3）＞异牡荆素（4）＞牡荆素（5），化合物1的抗氧化活性与合成抗氧化剂曲克芦丁相当或略强于曲克芦丁，且活性成分含量与抗氧化活性结果之间存在显著相关性。
本研究表明含羞草可能是天然抗氧化剂的潜在丰富来源。
