曼陀罗和狗牙根提取物的体外细胞毒性及抗氧化活性评估
Evaluation of In Vitro Cytotoxic and Antioxidant Activity of Datura metel Linn. and Cynodon dactylon Linn. Extracts.
作者信息
Roy Soumen, Pawar Sandip, Chowdhary Abhay
机构信息
Department of Virology, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, Maharashtra, India.
Department of Biochemistry, The Institute of Science, Fort, Mumbai, Maharashtra, India.
出版信息
Pharmacognosy Res. 2016 Apr-Jun;8(2):123-7. doi: 10.4103/0974-8490.175610.
AIM
To evaluate in vitro cytotoxicity and antioxidant activity of Datura metel L. and Cynodon dactylon L. extracts.
MATERIALS AND METHODS
The extraction of plants parts (datura seed and fruit pulp) and areal parts of durva was carried out using soxhlet and cold extraction method using solvents namely methanol and distilled water. The total phenolic content (TPC) and total flavonoid content (TFC) was determined by established methods. The in vitro cytotoxicity assay was performed in vero cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. In vitro antioxidant activity of the extract was performed by 2, 2-diphenyl-1-picrylhydrazyl radical scavenging method.
RESULTS
We found that the highest amount of TPC and TFC in methanolic extracts of seed (268.6 μg of gallic acid equivalence/mg of dry plant material) and fruit pulp (8.84 μg of quercetin equivalence/mg dry plant material) of D. metel, respectively prepared by Soxhlet method. The methanolic extract of C. dactylon prepared using soxhlation has shown potent free radical scavenging activity with 50% inhibitory concentration (IC50) value of 100 μg/ml. The IC50 of a methanolic cold extract of datura fruit was found to be 3 mg/ml against vero cell line.
CONCLUSION
We observed that plant parts of C. dactylon and D. metel have a high antioxidant activity. Further research is needed to explore the therapeutic potential of these plant extracts.
SUMMARY
In the present study we observed a positive correlation was between the phenolic and flavanoid content of the Datura metel and cynodon doctylon (durva) extracts with the free radical scavenging activities. Both were found to have a high antioxidant activity. Abbreviations used: BHA: Butylated hydroxyanisole, BHT: Butylated hydroxytoluene, CC50: 50% cell cytotoxic concentration, CNS: Central nervous system, DPPH: 2, 2-diphenyl-1-picrylhydrazyl, IC50: 50% inhibitory concentration, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), TFC: Total flavonoid content, TPC: Total phenolic content.
目的
评估曼陀罗和狗牙根提取物的体外细胞毒性和抗氧化活性。
材料与方法
采用索氏提取法和冷浸提取法，分别以甲醇和蒸馏水为溶剂，对曼陀罗的植物部位（种子和果肉）以及狗牙根的地上部分进行提取。通过既定方法测定总酚含量（TPC）和总黄酮含量（TFC）。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法在 vero 细胞系中进行体外细胞毒性试验。提取物的体外抗氧化活性通过 2,2-二苯基-1-苦基肼自由基清除法进行测定。
结果
我们发现，采用索氏提取法制备的曼陀罗种子甲醇提取物（268.6 μg 没食子酸当量/mg 干燥植物材料）和果肉甲醇提取物（8.84 μg 槲皮素当量/mg 干燥植物材料）中 TPC 和 TFC 含量最高。采用索氏提取法制备的狗牙根甲醇提取物表现出较强的自由基清除活性，50%抑制浓度（IC50）值为 100 μg/ml。曼陀罗果实甲醇冷提取物对 vero 细胞系的 IC50 为 3 mg/ml。
结论
我们观察到狗牙根和曼陀罗的植物部位具有较高的抗氧化活性。需要进一步研究以探索这些植物提取物的治疗潜力。
总结
在本研究中，我们观察到曼陀罗和狗牙根提取物的酚类和黄酮类含量与自由基清除活性之间存在正相关。两者均具有较高的抗氧化活性。使用的缩写：BHA：丁基羟基茴香醚，BHT：丁基羟基甲苯，CC50：50%细胞毒性浓度，CNS：中枢神经系统，DPPH：2,2-二苯基-1-苦基肼，IC50：50%抑制浓度，MTT：3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐，TFC：总黄酮含量，TPC：总酚含量。
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