Esmaeili N, Ebrahimzadeh H, Abdi K, Safarian S
Department of Plant Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Pharmacogn Mag. 2011 Jan;7(25):74-80. doi: 10.4103/0973-1296.75906.
It is well known that phenolic compounds are constituents of many plants. In this study, the total phenolics content in Crocus sativus L. corms in dormancy and waking stages were determined by the Folin-Ciocalteu method. Analysis was carried out by gas chromatography-mass spectrometry (GC-MS) after silylation by N-methyl-N-trimethylsilyl trifluroacetamide (MSTFA) + %1 trimethyl iodosilane (TMIS). Numerous compounds were detected and 11 compounds were identified. The highest phenolics content in waking corms was observed for gentisic acid (5.693 ± 0.057 μg/g) and the lowest for gallic acid (0.416 ± 0.006 μg/g); also these two phenolic compounds are the highest (0.929 ± 0.015 μg/g) and lowest (0.017 ± 0.001 μg/g) phenolics in dormant corms, respectively. The results from quantization and GC-MS analysis showed a high concentration of phenolic compounds in waking corms than the dormant stage. Furthermore, the radical scavenging activities of saffron corms were studied by 1,1-diphenyl-2-pycrylhydrazyl (DPPH) test and EC (50)values were determined about 2055 ppm and 8274 ppm for waking and dormant corms, respectively.
众所周知，酚类化合物是许多植物的组成成分。在本研究中，采用福林-西奥尔特法测定了藏红花球茎休眠期和苏醒期的总酚含量。通过N-甲基-N-三甲基硅烷基三氟乙酰胺（MSTFA）+1%三甲基碘硅烷（TMIS）进行硅烷化后，采用气相色谱-质谱联用仪（GC-MS）进行分析。检测到大量化合物，并鉴定出11种化合物。苏醒期球茎中酚酸含量最高的是龙胆酸（5.693±0.057μg/g），最低的是没食子酸（0.416±0.006μg/g）；这两种酚类化合物在休眠期球茎中也是含量最高（0.929±0.015μg/g）和最低（0.017±0.001μg/g）的酚类物质。定量分析和GC-MS分析结果表明，苏醒期球茎中酚类化合物的浓度高于休眠期。此外，通过1,1-二苯基-2-苦基肼（DPPH）试验研究了藏红花球茎的自由基清除活性，苏醒期和休眠期球茎的EC（50）值分别约为2055ppm和8274ppm。
