First Insights Into the LC-HRMS Profiling and Biological Activities of .

There are documented reports of 103 different species, commonly known as saffron, distributed across Türkiye. Many of these species have been used not only as spices but also in the treatment or prevention of various diseases. However, the majority of research has focused only on L., and only a few other members of the genus have been investigated for their chemical constituents and biological properties. Boiss. & Reut. is one of those species used against gynecological diseases through oral administration. This is the first report regarding phytochemicals by LC-HRMS analysis and in vitro biological activities of the species. Accordingly, a total of 33 different secondary metabolites were detected in varying quantities. The major compound was determined as rutin (162.808 ± 4.5 μg/g extract). Total phenolic content (32.79 ± 1.30 μg PEs/mg extract) and total flavonoid content (40.29 ± 0.24 μg QEs/mg) were calculated. The antioxidant activity of the extract was evaluated by three different assays, namely, DPPH free and ABTS cation radical scavenging and CUPRAC activity methods, and moderate activity was demonstrated compared to the standards. The extract inhibited acetylcholinesterase with 30.88% ± 1.51% and butyrylcholinesterase with 61.22% ± 0.47% at a 200 μg/mL concentration. Besides, the extract was effective against , , and strains. This study features a new contribution to research in Türkiye.