Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar 608 002, India.
J Biochem Mol Toxicol. 2011 Mar-Apr;25(2):60-7. doi: 10.1002/jbt.20359.
The present study aims to evaluate the protective effects of caffeic acid on isoproterenol-treated myocardial infarction. Male albino Wistar rats were pretreated with caffeic acid (15 mg/kg) daily for 10 days. After the pretreatment, rats were injected with isoproterenol (100 mg/kg) at an interval of 24 h for 2 days to induce myocardial infarction. Isoproterenol-treated rats showed increased intensity of lactate dehydrogenase-1 and 2 isoenzyme bands and elevated ST segments. The activity of the heart sodium potassium adenosine triphosphatase was decreased, and the activities of the heart magnesium adenosine triphosphatase and calcium adenosine triphosphatase were increased in isoproterenol-treated rats. Isoproterenol-treated rats also showed a significant increase in the concentration of heart calcium. Furthermore, it significantly increased the counts of red blood cells, hemoglobin, white blood cells, and neutrophils and decreased significantly the concentration of erythrocyte sedimentation rate and the counts of lymphocytes and eosinophils. Pretreatment with caffeic acid showed protective effects on all the biochemical parameters, hematology and minimized alterations in lactate dehydrogenase isoenzymes and electrocardiogram. In vitro study confirmed the free radical scavenging potential of caffeic acid. The observed effects might be due to the free radical scavenging and membrane-stabilizing property of caffeic acid.
本研究旨在评估咖啡酸对异丙肾上腺素诱导的心肌梗死的保护作用。雄性白化 Wistar 大鼠每日用咖啡酸（15mg/kg）预处理 10 天。预处理后，大鼠每隔 24 小时注射异丙肾上腺素（100mg/kg），连续 2 天诱导心肌梗死。异丙肾上腺素处理的大鼠显示乳酸脱氢酶-1 和 2 同工酶条带的强度增加，ST 段升高。心肌钠钾三磷酸腺苷酶的活性降低，而心肌镁三磷酸腺苷酶和钙三磷酸腺苷酶的活性增加。异丙肾上腺素处理的大鼠心脏内钙浓度也显著增加。此外，它还显著增加了红细胞、血红蛋白、白细胞和中性粒细胞的计数，而红细胞沉降率和淋巴细胞及嗜酸性粒细胞的计数则显著降低。咖啡酸预处理对所有生化参数、血液学参数以及乳酸脱氢酶同工酶和心电图的改变均有保护作用。体外研究证实了咖啡酸的自由基清除能力。观察到的作用可能归因于咖啡酸的自由基清除和膜稳定特性。
