State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, China.
Int J Mol Sci. 2022 Jul 5;23(13):7457. doi: 10.3390/ijms23137457.
has been reported to have potential therapeutic effects against myocardial ischemia. The pharmacological effects of oral natural medicines may be influenced by the participation of gut microbiota. In this study, we aimed to investigate the bidirectional regulation of gut microbiota and the main components of . We first established a quantitative method for the four main components (chlorogenic acid, syringin, acanthoside B, rutin) which were chosen by fingerprint using liquid chromatography tandem mass spectrometry (LC-MS/MS), and found that gut microbiota has a strong metabolic effect on them. Meanwhile, we identified five major rat gut microbiota metabolites (M1-M5) using liquid chromatography tandem time-of-flight mass spectrometry (LC/MS-IT-TOF). The metabolic properties of metabolites in vitro were preliminarily elucidated by LC-MS/MS for the first time. These five metabolites of may all have potential contributions to the treatment of myocardial ischemia. Furthermore, the four main components (10 μg/mL) can significantly stimulate intestinal bacteria to produce short chain fatty acids in vitro, respectively, which can further contribute to the effect in myocardial ischemia. In this study, the therapeutic effect against myocardial ischemia of was first reported to be related to the intestinal flora, which can be useful in understanding the effective substances of .
已报道其具有抗心肌缺血的潜在治疗作用。口服天然药物的药理作用可能受到肠道微生物群的参与的影响。在这项研究中，我们旨在研究肠道微生物群和 的主要成分的双向调节。我们首先使用液相色谱串联质谱（LC-MS/MS）建立了一种定量方法，用于选择的四个主要成分（绿原酸、獐牙菜苷、獐牙菜苦苷 B、芦丁），并发现肠道微生物群对它们具有很强的代谢作用。同时，我们使用液相色谱串联飞行时间质谱（LC/MS-IT-TOF）鉴定了五种主要的大鼠肠道微生物群代谢物（M1-M5）。首次通过 LC-MS/MS 初步阐明了代谢物的代谢特性。 的这些五种代谢物可能都对治疗心肌缺血有潜在贡献。此外，四种主要成分（10μg/mL）可分别显著刺激肠道细菌在体外产生短链脂肪酸，这可进一步有助于心肌缺血的作用。在这项研究中， 对心肌缺血的治疗作用首先被报道与肠道菌群有关，这有助于了解 的有效物质。
