体内多焦点光分辨光声显微镜。
Multifocal optical-resolution photoacoustic microscopy in vivo.
机构信息
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, Missouri 63130, USA.
出版信息
Opt Lett. 2011 Apr 1;36(7):1236-8. doi: 10.1364/OL.36.001236.
Abstract
Although ultrasound arrays have been exploited in photoacoustic imaging to improve imaging speed, ultrasound-array-based optical-resolution photoacoustic microscopy (OR-PAM) has never been achieved previously to our knowledge. Here we present our development of multifocal OR-PAM using a microlens array for optical illumination and an ultrasound array for photoacoustic detection. Our system is capable of imaging hemoglobin concentration and oxygenation in individual microvessels in vivo at high speed. Compared with a single focus, multiple foci reduce the scanning load and increase the imaging speed significantly. The current multifocal system can acquire 1000 × 500 × 200 voxels at ~10 μm lateral resolution within 4 min.
摘要
虽然超声阵列已被应用于光声成象以提高成像速度，但据我们所知，以前从未实现过基于超声阵列的光学分辨率光声显微镜（OR-PAM）。在这里，我们展示了使用微透镜阵列进行光学照明和超声阵列进行光声检测的多焦点 OR-PAM 的发展。我们的系统能够以高速对体内单个微血管中的血红蛋白浓度和氧合进行成像。与单个焦点相比，多个焦点显著降低了扫描负荷并提高了成像速度。当前的多焦点系统可以在 4 分钟内以约 10μm 的横向分辨率获取 1000×500×200 个体素。
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