Department of Thoracic Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, China.
The Fourth Clinical College of Nanjing Medical University, Nanjing, China.
Thorac Cancer. 2019 Apr;10(4):909-917. doi: 10.1111/1759-7714.13027. Epub 2019 Mar 5.
FLOT1 is a scaffolding protein of lipid rafts that is believed to be involved in numerous cellular processes. However, few studies have explored the function of FLOT1 in the development of lung adenocarcinoma (LUAD) and the underlying mechanisms of FLOT1 activity.
FLOT1 knockdown and overexpression models were constructed via lentivirus. Cell growth, invasion, migration, and apoptosis were detected to evaluate the role of FLOT1 in LUAD development. Epithelial-mesenchymal transition (EMT) and cell cycle regulatory markers were then examined. Finally, the influence of FLOT1 on the Erk/Akt signaling pathway was investigated.
FLOT1 promoted cell growth, invasion, and migration and inhibited cell apoptosis. In addition, FLOT1 induced EMT and modulated the cell cycle by activating the Erk/Akt signaling pathway.
The findings indicate a significant role of FLOT1 in LUAD development. Targeting FLOT1 may be a potential therapeutic strategy for LUAD.
FLOT1 是脂筏的支架蛋白，被认为参与了许多细胞过程。然而，很少有研究探讨 FLOT1 在肺腺癌（LUAD）发展中的作用及其 FLOT1 活性的潜在机制。
通过慢病毒构建了 FLOT1 敲低和过表达模型。通过检测细胞生长、侵袭、迁移和凋亡来评估 FLOT1 在 LUAD 发展中的作用。然后检查上皮-间充质转化（EMT）和细胞周期调节标志物。最后，研究了 FLOT1 对 Erk/Akt 信号通路的影响。
FLOT1 促进了细胞生长、侵袭和迁移，抑制了细胞凋亡。此外，FLOT1 通过激活 Erk/Akt 信号通路诱导 EMT 并调节细胞周期。
这些发现表明 FLOT1 在 LUAD 发展中起着重要作用。靶向 FLOT1 可能是 LUAD 的一种潜在治疗策略。
