Laboratoire de Virologie, Assistance Publique-Hôpitaux de Paris (AP-HP), Hôpital Européen Georges Pompidou, Université Paris Descartes, Paris 5, 20 rue Leblanc, 75015 Paris, France.
Arch Virol. 2011 Sep;156(9):1603-6. doi: 10.1007/s00705-011-0997-9. Epub 2011 Apr 11.
We compared paired plasma and dried blood spot (DBS) samples from 54 HIV-1-treated children living in Bangui, Central African Republic, for antiretroviral-resistance-associated mutations. All children displayed virological failure (HIV-1 RNA >3.70 log(10)copies/ml). Testing for resistance genotype was carried out in a reference laboratory in Paris, France. A successful test result was obtained in 54 (100%) plasmas and 25 DBSs (46%). Among the 732 resistance-associated mutations analyzed, 718 were identical, leading to a high concordance rate of 98.1%. Genotypic resistance tests on DBS samples were found to be highly feasible and accurate in a foreign reference laboratory, but with additional costs for shipping and decreased sensitivity.
我们比较了来自中非共和国班吉 54 名接受 HIV-1 治疗的儿童的配对血浆和干血斑（DBS）样本，以检测与抗逆转录病毒耐药性相关的突变。所有儿童均表现出病毒学失败（HIV-1 RNA >3.70 log(10)拷贝/ml）。耐药基因型检测在法国巴黎的一个参考实验室进行。54 份（100%）血浆和 25 份 DBS（46%）成功进行了检测。在分析的 732 个与耐药性相关的突变中，718 个是相同的，导致高度一致率为 98.1%。在外国参考实验室，DBS 样本的基因耐药性检测非常可行且准确，但运输成本增加，灵敏度降低。
