Henry Jackson Foundation, MHRP-HJF, Silver Spring, Maryland, USA
Henry Jackson Foundation, MHRP-HJF, Silver Spring, Maryland, USA.
J Clin Microbiol. 2018 Sep 25;56(10). doi: 10.1128/JCM.00933-18. Print 2018 Oct.
Dried blood spots (DBS) are frequently used in clinical testing for biosurveillance, infectious disease and confirmatory testing, and clinical trials, particularly for populations in remote areas. The HemaSpot-HF blood collection device (HS) provides an alternative format to the Whatman 903 cards (903) to simplify sample collection and processing. In this study, the performance of the HS was compared to that of the 903 using previously characterized clinical specimens and HIV seroconversion panels known to exhibit markers of early human immunodeficiency virus (HIV) infection. HS and 903 samples were prepared and tested by Bio-Rad GS HIV Combo Ag/Ab enzyme immunoassay (EIA), GS HIV-1/-2 Plus O EIA, GS HIV-1 Western blot, and HIV-1 Geenius assays. Both HS and 903 performed well for up to 6 months at room temperature, but a marked loss of Western blot and low titer antibody signals from early infection samples was observed in samples stored for 180 days at elevated (37 to 45°C) temperatures and high humidity (95%). HemaSpot samples placed in sealed bags with additional desiccant were protected from degradation and showed improved signal recovery relative to that of the 903. HS was easier to use than the 903 and showed higher sensitivity and reproducibility for early infection samples and improved stability.
干血斑（DBS）常用于临床检测中的生物监测、传染病和确证检测以及临床试验，特别是在偏远地区的人群中。HemaSpot-HF 血液采集装置（HS）提供了一种替代 Whatman 903 卡（903）的格式，可简化样本采集和处理。在这项研究中，HS 的性能与 903 进行了比较，使用了先前经过特征描述的临床标本和已知表现出早期人类免疫缺陷病毒（HIV）感染标志物的 HIV 血清转换面板。HS 和 903 样本通过 Bio-Rad GS HIV Combo Ag/Ab 酶免疫分析（EIA）、GS HIV-1/-2 Plus O EIA、GS HIV-1 Western blot 和 HIV-1 Geenius 检测进行了制备和测试。在室温下，HS 和 903 都能在长达 6 个月的时间内表现良好，但在升高的（37 至 45°C）温度和高湿度（95%）下储存 180 天时，Western blot 和早期感染样本低滴度抗体信号明显丢失。密封袋中放置的 HemaSpot 样本并添加干燥剂可防止降解，并显示出比 903 更好的信号恢复。HS 比 903 更易于使用，对早期感染样本具有更高的灵敏度和重现性，并且稳定性更好。
