Pharmacology Research Laboratory, Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
Phytother Res. 2011 Dec;25(12):1806-12. doi: 10.1002/ptr.3491. Epub 2011 Apr 8.
The aim of the present study was to evaluate the efficacy of a standardized methanol extract of Quassia amara L. (Family: Simaroubaceae) in nicotinamide-streptozotocin-induced diabetic rats. Non insulin dependent diabetes mellitus was induced by streptozotocin in rats pre-treated with nicotinamide. Diabetic rats were treated with oral doses of Quassia amara extract (QaE; 100 and 200 mg/kg) or glibenclamide (10 mg/kg; as standard). QaE and glibenclamide were administered as a suspension in 0.3% carboxy methyl cellulose for 14 days. Control animals received an equal volume of vehicle. Blood samples were collected by retro-orbital puncture on day 14, 1 h after last treatment. Plasma glucose, insulin and lipid parameters (total cholesterol, LDL-C, HDL-C and triglycerides) were measured using commercially available biochemical kits. The oral glucose tolerance test was performed to evaluate the effect of the extract on peripheral glucose utilization in normal rats. Both doses of QaE significantly (p < 0.01) reduced elevated fasting blood glucose levels in diabetic rats. In the oral glucose tolerance test, QaE treatment significantly increased (p < 0.05) the glucose tolerance compared with the vehicle. QaE and glibenclamide, effectively normalized dyslipidemia associated with streptozotocin-induced diabetes. The findings of the present study indicate that Quassia amara extract may be potentially valuable in the treatment of diabetes and associated dyslipidemia.
本研究旨在评估金鸡纳树（Simaroubaceae 科）甲醇标准化提取物在烟酰胺-链脲佐菌素诱导的糖尿病大鼠中的疗效。非胰岛素依赖型糖尿病通过链脲佐菌素在预先用烟酰胺预处理的大鼠中诱导。糖尿病大鼠给予金鸡纳树提取物（QaE；100 和 200mg/kg）或格列本脲（10mg/kg；作为标准）的口服剂量。QaE 和格列本脲以 0.3%羧甲基纤维素中的混悬液形式给药，共 14 天。对照动物接受等量的载体。在第 14 天，末次处理后 1 小时，通过眼眶后穿刺收集血样。使用市售的生化试剂盒测量血浆葡萄糖、胰岛素和脂质参数（总胆固醇、LDL-C、HDL-C 和甘油三酯）。进行口服葡萄糖耐量试验以评估提取物对正常大鼠外周葡萄糖利用的影响。两种剂量的 QaE 均显著（p<0.01）降低糖尿病大鼠升高的空腹血糖水平。在口服葡萄糖耐量试验中，与载体相比，QaE 治疗显著增加（p<0.05）葡萄糖耐量。QaE 和格列本脲有效纠正了链脲佐菌素诱导的糖尿病相关的血脂异常。本研究的结果表明，金鸡纳树提取物在治疗糖尿病和相关血脂异常方面可能具有潜在价值。
