Department of Chemistry, University of Sheffield, Sheffield, Yorkshire, UK.
Biomacromolecules. 2011 Jun 13;12(6):2225-34. doi: 10.1021/bm200311s. Epub 2011 May 2.
Facile derivatization of rhodamine 6G in the 2' position by direct reaction with secondary amines is reported. If the secondary amine contains a hydroxy group, the hydroxyl-functional intermediate can be readily esterified to give either fluorescent initiators for atom transfer radical polymerization (ATRP) or a fluorescent methacrylic comonomer. In contrast to rhodamine dyes functionalized using primary amines, which are only fluorescent at low pH, these compounds are highly fluorescent at physiological pH. These new compounds were subsequently used to prepare a range of fluorescently labeled biocompatible polymers based on the biomimetic monomer, 2-(methacryloyloxy)ethyl phosphorylcholine (MPC), for biomedical studies.
报道了通过与仲胺的直接反应在 2' 位上方便地衍生化罗丹明 6G。如果仲胺含有羟基，则羟基官能中间体可以很容易酯化，得到用于原子转移自由基聚合（ATRP）的荧光引发剂或荧光甲基丙烯酸酯共聚单体。与使用伯胺官能化的罗丹明染料不同，这些化合物在低 pH 值下仅具有荧光，而在生理 pH 值下具有很强的荧光。这些新化合物随后用于根据仿生单体 2-（甲基丙烯酰氧基）乙基膦酸（MPC）制备一系列荧光标记的生物相容性聚合物，用于生物医学研究。
