Kapil Kriti, Xu Shirley, Lee Inseon, Murata Hironobu, Kwon Seok-Joon, Dordick Jonathan S, Matyjaszewski Krzysztof
Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA.
Department of Chemical and Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Polymers (Basel). 2023 Jun 18;15(12):2723. doi: 10.3390/polym15122723.
Infectious diseases caused by pathogens are a health burden, but traditional pathogen identification methods are complex and time-consuming. In this work, we have developed well-defined, multifunctional copolymers with rhodamine B dye synthesized by atom transfer radical polymerization (ATRP) using fully oxygen-tolerant photoredox/copper dual catalysis. ATRP enabled the efficient synthesis of copolymers with multiple fluorescent dyes from a biotin-functionalized initiator. Biotinylated dye copolymers were conjugated to antibody (Ab) or cell-wall binding domain (CBD), resulting in a highly fluorescent polymeric dye-binder complex. We showed that the unique combination of multifunctional polymeric dyes and strain-specific Ab or CBD exhibited both enhanced fluorescence and target selectivity for bioimaging of by flow cytometry and confocal microscopy. The ATRP-derived polymeric dyes have the potential as biosensors for the detection of target DNA, protein, or bacteria, as well as bioimaging.
由病原体引起的传染病是一种健康负担，但传统的病原体鉴定方法复杂且耗时。在这项工作中，我们利用完全耐氧的光氧化还原/铜双催化通过原子转移自由基聚合（ATRP）合成了带有罗丹明B染料的定义明确的多功能共聚物。ATRP能够从生物素功能化引发剂高效合成带有多种荧光染料的共聚物。生物素化的染料共聚物与抗体（Ab）或细胞壁结合结构域（CBD）偶联，形成高度荧光的聚合物染料-结合剂复合物。我们表明，多功能聚合物染料与菌株特异性Ab或CBD的独特组合在通过流式细胞术和共聚焦显微镜对[此处原文缺失具体检测对象]进行生物成像时表现出增强的荧光和目标选择性。ATRP衍生的聚合物染料有潜力作为生物传感器用于检测目标DNA、蛋白质或细菌以及生物成像。
