Department of Chemistry, Chonbuk National University, Jeonju 561-756, South Korea.
Talanta. 2011 May 15;84(3):752-8. doi: 10.1016/j.talanta.2011.02.001. Epub 2011 Feb 25.
A novel, quantitative analytical method for measuring C-reactive protein (CRP) levels in human serum has been developed based on the catalytic activity of gold nanoparticles (GNPs) and luminol-H(2)O(2) chemiluminescence (CL). The CL intensity in the presence of CRP and its ligand, O-phosphorylethanolamine (PEA), was greatly enhanced due to the aggregation of GNPs after the addition of 0.5M NaCl. Any pretreatment steps, such as covalent functionalization of GNPs, addition of antibodies, or labeling of CRP, were not needed for CL detection. The CL enhancement was linearly proportional to CRP concentration in the range of 1.88 fM to 1.925 pM. The detection limit of CRP in serum samples was estimated to be as low as 1.88 fM. The detection sensitivity was increased more than 164 times of magnitude over that of the conventional, enzyme-linked immunosorbent assay (ELISA) method. This proposed GNP-based CL detection method offers the advantages of simplicity, rapidity, and sensitivity.
基于金纳米粒子（GNPs）和鲁米诺-H₂O₂化学发光（CL）的催化活性，开发了一种新型、定量分析人血清中 C 反应蛋白（CRP）水平的方法。在存在 CRP 及其配体 O-磷酸乙醇胺（PEA）的情况下，由于添加 0.5M NaCl 后 GNPs 的聚集，CL 强度大大增强。对于 CL 检测，不需要进行任何预处理步骤，例如 GNPs 的共价功能化、抗体的添加或 CRP 的标记。CL 增强与 CRP 浓度在 1.88 fM 至 1.925 pM 的范围内呈线性比例关系。在血清样品中检测 CRP 的检测限估计低至 1.88 fM。与传统的酶联免疫吸附测定（ELISA）方法相比，检测灵敏度提高了 164 多倍。这种基于 GNP 的 CL 检测方法具有简单、快速和灵敏的优点。
