Geng Ying, Schery Lee Anne, Sharma Robin, Dubra Alfredo, Ahmad Kamran, Libby Richard T, Williams David R
Biomed Opt Express. 2011 Feb 28;2(4):717-38. doi: 10.1364/BOE.2.000717.
The Shack-Hartmann wavefront sensor (SHWS) spots upon which ocular aberration measurements depend have poor quality in mice due to light reflected from multiple retinal layers. We have designed and implemented a SHWS that can favor light from a specific retinal layer and measured monochromatic aberrations in 20 eyes from 10 anesthetized C57BL/6J mice. Using this instrument, we show that mice are myopic, not hyperopic as is frequently reported. We have also measured longitudinal chromatic aberration (LCA) of the mouse eye and found that it follows predictions of the water-filled schematic mouse eye. Results indicate that the optical quality of the mouse eye assessed by measurement of its aberrations is remarkably good, better for retinal imaging than the human eye. The dilated mouse eye has a much larger numerical aperture (NA) than that of the dilated human eye (0.5 NA vs. 0.2 NA), but it has a similar amount of root mean square (RMS) higher order aberrations compared to the dilated human eye. These measurements predict that adaptive optics based on this method of wavefront sensing will provide improvements in retinal image quality and potentially two times higher lateral resolution than that in the human eye.
由于存在来自多个视网膜层的反射光，用于测量眼像差的夏克-哈特曼波前传感器（SHWS）在小鼠眼中的光斑质量较差。我们设计并实现了一种能够优先采集特定视网膜层光线的SHWS，并对10只麻醉状态下的C57BL/6J小鼠的20只眼睛进行了单色像差测量。使用该仪器，我们发现小鼠是近视的，而非如经常报道的那样是远视。我们还测量了小鼠眼睛的纵向色差（LCA），发现其符合充满水的示意性小鼠眼睛的预测。结果表明，通过测量像差评估的小鼠眼睛的光学质量非常好，对于视网膜成像而言比人眼更好。散瞳后的小鼠眼睛的数值孔径（NA）比散瞳后的人眼大得多（0.5 NA对0.2 NA），但与散瞳后的人眼相比，其均方根（RMS）高阶像差量相似。这些测量结果预测，基于这种波前传感方法的自适应光学将改善视网膜图像质量，并可能提供比人眼高两倍的横向分辨率。
