肝内自然杀伤 T 细胞群体在人类肝脂肪变性中增加。
Intrahepatic natural killer T cell populations are increased in human hepatic steatosis.
机构信息
New York University School of Medicine, New York, NY 10016, USA.
出版信息
World J Gastroenterol. 2011 Apr 7;17(13):1725-31. doi: 10.3748/wjg.v17.i13.1725.
AIM
To determine if natural killer T cell (NKT) populations are affected in nonalcoholic fatty liver disease (NAFLD).
METHODS
Patients undergoing bariatric surgery underwent liver biopsy and blood sampling during surgery. The biopsy was assessed for steatosis and immunocyte infiltration. Intrahepatic lymphocytes (IHLs) were isolated from the remainder of the liver biopsy, and peripheral blood mononuclear cells (PBMCs) were isolated from the blood. Expression of surface proteins on both IHLs and PBMCs were quantified using flow cytometry.
RESULTS
Twenty-seven subjects participated in this study. Subjects with moderate or severe steatosis had a higher percentage of intrahepatic CD3+/CD56+ NKT cells (38.6%) than did patients with mild steatosis (24.1%, P = 0.05) or those without steatosis (21.5%, P = 0.03). Patients with moderate to severe steatosis also had a higher percentage of NKT cells in the blood (12.3%) as compared to patients with mild steatosis (2.5% P = 0.02) and those without steatosis (5.1%, P = 0.05).
CONCLUSION
NKT cells are significantly increased in the liver and blood of patients with moderate to severe steatosis and support the role of NKT cells in NAFLD.
目的
确定自然杀伤 T 细胞 (NKT) 群体是否在非酒精性脂肪性肝病 (NAFLD) 中受到影响。
方法
接受减肥手术的患者在手术期间进行肝活检和采血。评估活检标本的脂肪变性和免疫细胞浸润情况。从剩余的肝活检组织中分离出肝内淋巴细胞 (IHL)，从血液中分离外周血单核细胞 (PBMC)。使用流式细胞术定量测定 IHL 和 PBMC 表面蛋白的表达。
结果
27 名受试者参与了这项研究。中重度脂肪变性患者肝内 CD3+/CD56+ NKT 细胞（38.6%）的比例高于轻度脂肪变性患者（24.1%，P=0.05）或无脂肪变性患者（21.5%，P=0.03）。中重度脂肪变性患者血液中 NKT 细胞的比例（12.3%）也高于轻度脂肪变性患者（2.5%，P=0.02）和无脂肪变性患者（5.1%，P=0.05）。
结论
中重度脂肪变性患者肝内和血液中的 NKT 细胞明显增加，支持 NKT 细胞在 NAFLD 中的作用。
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