Int J Oral Maxillofac Implants. 2011 Mar-Apr;26(2):274-81.
The purpose of this study was twofold: (1) to estimate the level of microbial contamination found on commercial guides (CG) and in-house laboratory guides (LG) prior to use, and (2) to evaluate the antimicrobial potential of disinfectants commonly used in dentistry to decontaminate heat-sensitive surgical guides. Ethylene oxide gas was used as the positive control; sterile water served as a negative control.
Evaluation of CGs and LGs for bacterial contamination occurred soon after their arrival in the laboratory. Some guides went directly into tubes of trypticase soy broth solution, vortexed and equally divided into two tubes. One tube went into an 80°C bath for 19 minutes, while the other stayed at room temperature. After plating, half of the samples underwent anaerobic incubation. All incubation was for 48 hours at 37°C. Other guides underwent water rinsing or disinfection by various methods for 5 or 15 minutes or ethylene oxide gas sterilization prior to sampling.
Untreated CG specimens showed modest levels of bacterial contamination, with most colonies coming from liquid specimens not exposed to 80°C. LG specimens had more bacteria from both heat-treated and non-heat-treated aliquots. Chlorhexidine gluconate, diluted bleach, and water rinsing were not able to completely eliminate microorganisms on the specimens, but no viable microorganisms were present on specimens treated with gas sterilization or 70% ethanol for 15 minutes.
CGs and LGs, on average, had different levels of bacterial contamination prior to disinfection. Water, chlorhexidine gluconate, and diluted bleach were not totally successful in decontaminating the surgical guides, but no growth was found after 15 minutes of immersion in 70% ethanol. Preferably, surgical guides should be submerged in 70% alcohol for a minimum of 15 minutes or undergo sterilization using ethylene oxide gas.
本研究有两个目的：（1）评估使用前商用导板（CG）和内部实验室导板（LG）上发现的微生物污染程度，（2）评估牙科中常用的消毒剂对热敏感手术导板进行消毒的抗菌潜力。环氧乙烷气体用作阳性对照；无菌水作为阴性对照。
在导板到达实验室后不久，就对 CG 和 LG 进行了细菌污染评估。一些导板直接放入胰蛋白酶大豆肉汤溶液管中，涡旋并等分为两管。一管放入 80°C 水浴中 19 分钟，另一管保持室温。培养后，一半样本进行厌氧孵育。所有孵育均在 37°C 下进行 48 小时。其他导板在采样前经过各种方法的水冲洗或消毒 5 或 15 分钟或环氧乙烷气体灭菌。
未经处理的 CG 标本显示出适度的细菌污染水平，大多数菌落来自未暴露于 80°C 的液体标本。LG 标本中，来自热处理和非热处理等分样的细菌更多。洗必泰葡萄糖酸盐、稀释漂白剂和水冲洗都不能完全消除标本上的微生物，但经过气体灭菌或 70%乙醇处理 15 分钟的标本上没有存活的微生物。
在消毒前，CG 和 LG 的细菌污染程度平均不同。水、洗必泰葡萄糖酸盐和稀释漂白剂在消毒手术导板方面不完全成功，但在 70%乙醇中浸泡 15 分钟后未发现生长。最好将手术导板浸泡在 70%酒精中至少 15 分钟或使用环氧乙烷气体进行灭菌。
