Rhode Island Shoulder and Elbow Service, Brown Alpert Medical School, 2 Dudley Street, Suite 200, Providence, Rhode Island 02905, USA.
J Orthop Res. 2011 Nov;29(11):1695-9. doi: 10.1002/jor.21416. Epub 2011 Apr 11.
Chemokines produced by synoviocytes of the subacromial bursa are up-regulated in subacromial bursitis and rotator cuff disease. We hypothesized that SDF-1α production in bursal synoviocytes may be induced by local cytokines such as interleukin IL-1β and IL-6. Subacromial bursa specimens were obtained from patients undergoing shoulder surgery. Bursal specimens were stained with anti-human antibodies to IL-1, IL-6, and SDF-1α by immunohistochemistry and compared to normal and rheumatoid controls. Bursal cells were also isolated from specimens and cultured. Early passaged cells were then treated with cytokines (IL-1β and IL-6) and SDF-1α expression was measured by ELISA and RT-PCR. SDF-1α, IL-1β, and IL-6 were expressed at high levels in bursitis specimens from human subacromial bursa compared to normal controls. In cultured bursal synoviocytes, there was a dose-dependent increase in SDF-1α production in the supernatants of cells treated with IL-1β. SDF-1α mRNA expression was also increased in bursal cells treated with IL-1β. IL-6 caused a minimal but not statistically significant increase in SDF-1α expression. SDF-1α, IL-1β, and IL-6 are expressed in the inflamed human subacromial bursal tissues in patients with subacromial bursitis. In cultured bursal synoviocytes, SDF-1α gene expression and protein production are stimulated by IL-1β. IL-1β produced by bursal syvoviocytes and inflammatory cells in the human subacromial bursa is an important signal in the inflammatory response that occurs in subacromial bursitis and rotator cuff disease.
滑液囊中滑膜细胞产生的趋化因子在肩峰下滑囊炎和肩袖疾病中上调。我们假设滑液囊中滑膜细胞 SDF-1α 的产生可能是由局部细胞因子如白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）诱导的。我们从接受肩部手术的患者中获取肩峰下滑囊标本。通过免疫组织化学法用抗人 IL-1、IL-6 和 SDF-1α 的抗体对滑囊标本进行染色，并与正常和类风湿对照进行比较。还从标本中分离滑液囊细胞并进行培养。然后用细胞因子（IL-1β 和 IL-6）处理早期传代细胞，并通过 ELISA 和 RT-PCR 测量 SDF-1α 的表达。与正常对照组相比，来自人类肩峰下滑囊的滑囊炎标本中 SDF-1α、IL-1β 和 IL-6 的表达水平较高。在培养的滑液囊中，用 IL-1β 处理的细胞上清液中 SDF-1α 的产生呈剂量依赖性增加。用 IL-1β 处理的滑液囊中 SDF-1α mRNA 的表达也增加了。IL-6 引起的 SDF-1α 表达增加虽小但无统计学意义。SDF-1α、IL-1β 和 IL-6 在患有肩峰下滑囊炎的患者的发炎的人类肩峰下滑囊中表达。在培养的滑液囊中，SDF-1α 基因表达和蛋白产生受到 IL-1β 的刺激。滑液囊中滑膜细胞和人类肩峰下滑囊中的炎症细胞产生的 IL-1β 是发生在肩峰下滑囊炎和肩袖疾病中的炎症反应中的重要信号。
