石墨烯对正常人体肺细胞（BEAS-2B）的毒性。

Toxicity of graphene in normal human lung cells (BEAS-2B).

机构信息

Nanomaterial Toxicology Group, Indian Institute of Toxicology Research (CSIR), P.O. Box 80, M. G. Marg, Lucknow 226001, India.

出版信息

J Biomed Nanotechnol. 2011 Feb;7(1):106-7. doi: 10.1166/jbn.2011.1224.

DOI:10.1166/jbn.2011.1224

PMID:21485826

Abstract

Graphite nanomaterials such as thermally exfoliated graphite oxide (GO) are versatile in many applications. However, little is known about its effects on biological systems. In this study we characrerized the GO using dynamic light scattering (DLS) along with the toxicological aspects related to cytotoxicity and apoptosis in normal human lung cells (BEAS-2B). A significant concentration and time dependent decrease in cell viability was observed at different concentrations (10-100 microg/ml) by the MTT assay after 24 and 48 h of exposure and significant increase of early and late apoptotic cells was observed as compared to control cells. Our study demonstrates that GO induces cytotoxicity and apoptosis in human lung cells.

摘要

石墨纳米材料，如热剥离氧化石墨（GO），在许多应用中具有多功能性。然而，关于其对生物系统的影响知之甚少。在这项研究中，我们使用动态光散射（DLS）对 GO 进行了表征，并研究了其对正常人类肺细胞（BEAS-2B）的细胞毒性和细胞凋亡相关的毒理学方面。通过 MTT 测定，在暴露 24 和 48 小时后，在不同浓度（10-100 微克/毫升）下观察到细胞活力显著且浓度和时间依赖性下降，与对照细胞相比，早期和晚期凋亡细胞的数量显著增加。我们的研究表明，GO 可诱导人肺细胞的细胞毒性和细胞凋亡。