Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, United States.
Org Lett. 2011 May 6;13(9):2298-301. doi: 10.1021/ol2004885. Epub 2011 Apr 12.
Ketone handedness was discriminated using circular dichroism (CD) spectroscopy by monitoring the metal-to-ligand charge transfer (MLCT) bands of complexes between [Cu(I)((S)-1)(CH(3)CN)(2)]PF(6) and derivatized α-chiral cyclohexanones (4). This method was able to quantify the enantiomeric excess of unknown samples using a calibration curve, giving an absolute error of ±7%. The analysis was fast, allowing potential application of this assay in high-throughput screening (HTS).
酮手性通过监测 [Cu(I)((S)-1)(CH(3)CN)(2)]PF(6) 与衍生化的 α-手性环己酮 (4) 之间配合物的金属-配体电荷转移 (MLCT) 带，使用圆二色性 (CD) 光谱进行区分。该方法能够使用校准曲线定量未知样品的对映体过量，绝对误差为 ±7%。分析速度快，允许该测定法在高通量筛选 (HTS) 中潜在应用。
