Dragna Justin M, Gade Alexandra M, Tran Lee, Lynch Vince M, Anslyn Eric V
Water Lens LLC, Houston, Texas.
Chirality. 2015 Apr;27(4):294-8. doi: 10.1002/chir.22426. Epub 2015 Feb 9.
A receptor assembly composed of iron(II) triflate and pyridine-2,6-dicarbaldehyde was used to determine the enantiomeric excess (ee) of alpha-chiral primary amines using circular dichroism spectroscopy. The alpha chiral amines condense with the dialdehyde to form a diimine, which forms a 2:1 octahedral complex with iron(II). The ee values of unknown concentrations of alpha-chiral amines were determined by constructing calibration curves for each amine and then measuring the ellipticity at 600 nm. This improves our previously reported assay for ee determination of chiral primary amines by further increasing the wavelength at which CD is measured and reducing the absolute error of the assay.
由三氟甲磺酸铁（II）和2,6-吡啶二甲醛组成的受体组件用于通过圆二色光谱法测定α-手性伯胺的对映体过量（ee）。α-手性胺与二醛缩合形成二亚胺，该二亚胺与铁（II）形成2:1的八面体配合物。通过为每种胺构建校准曲线，然后在600nm处测量椭圆率，确定未知浓度的α-手性胺的ee值。通过进一步提高测量圆二色性的波长并降低测定的绝对误差，这改进了我们先前报道的用于手性伯胺ee测定的方法。
