Nieto Sonia, Lynch Vincent M, Anslyn Eric V, Kim Hyunwoo, Chin Jik
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, Texas 78712, USA.
J Am Chem Soc. 2008 Jul 23;130(29):9232-3. doi: 10.1021/ja803443j. Epub 2008 Jun 24.
This manuscript describes a protocol for the fast determination of identity, enantiomeric excess (ee) and concentration of chiral 1,2-diamines, the combination of which has not previously been achieved, using the changes in the circular dichroism (CD) spectra of the charge-transfer bands of the Cu(I)-Binap complex. The spectra were analyzed with a variety of pattern recognition (PR) protocols. PR techniques were used to analyze unknown samples in a robotically controlled 96-well plate interfaced CD instrument. In less than two minutes per sample, ee and concentration values can be read with 2% and 8% error, respectively. The speed and accuracy as well as ability to simultaneously measure ee concentration and identity represent clear advantages over traditional methods and makes this method adaptable to true high-throughput screening.
本手稿描述了一种使用Cu(I)-联萘二膦配合物电荷转移带的圆二色性(CD)光谱变化快速测定手性1,2-二胺的身份、对映体过量(ee)和浓度的方法,此前尚未实现这些参数的同时测定。使用多种模式识别(PR)协议对光谱进行分析。PR技术用于在机器人控制的96孔板接口CD仪器中分析未知样品。每个样品不到两分钟,就可以分别以2%和8%的误差读取ee和浓度值。与传统方法相比,该方法的速度、准确性以及同时测量ee、浓度和身份的能力具有明显优势,使其适用于真正的高通量筛选。