Department of Plant and Environmental Sciences, Norwegian University of Life Sciences, Aas, Norway.
Nanotoxicology. 2012 Mar;6(2):186-95. doi: 10.3109/17435390.2011.569094. Epub 2011 Apr 13.
Due to difficulties in tracing engineered nanoparticles (ENPs) in complex media, there are few data on the exposure of soil biota to ENPs. This study used neutron activated cobalt (Co NPs) and silver (Ag NPs) nanoparticles, as well as soluble cobalt and silver salts, to assess the uptake, excretion and biodistribution in the earthworm Eisenia fetida. Concentrations of cobalt in worms after four weeks exposure reached 88% and 69% of the Co ions and Co NPs concentrations in food, respectively, while corresponding values for Ag ions and Ag NPs were 2.3% and 0.4%. Both Ag ions and Ag NPs in earthworms were excreted rapidly, while only 32% of the cobalt accumulated from Co ions and Co NPs were excreted within four months. High accumulation of cobalt was found in blood and in the digestive tract. Metal characterization in the exposure medium was assessed by sequential extraction and ultrafiltration. The Co NPs showed significant dissolution and release of ions, while Ag ions and particularly Ag NPs were more inert.
由于在复杂介质中追踪工程纳米粒子（ENPs）存在困难，因此关于土壤生物群暴露于 ENPs 的数据很少。本研究使用中子激活的钴（Co NPs）和银（Ag NPs）纳米粒子以及可溶性钴和银盐，评估了蚯蚓（Eisenia fetida）对其的吸收、排泄和体内分布。在四周暴露后，蚯蚓体内的钴浓度分别达到食物中 Co 离子和 Co NPs 浓度的 88%和 69%，而 Ag 离子和 Ag NPs 的相应值分别为 2.3%和 0.4%。Ag 离子和 Ag NPs 在蚯蚓体内迅速排泄，而从 Co 离子和 Co NPs 积累的钴中只有 32%在四个月内排泄。在血液和消化道中发现了钴的高积累。通过连续提取和超滤评估了暴露介质中的金属特性。Co NPs 表现出明显的溶解和离子释放，而 Ag 离子特别是 Ag NPs 则更惰性。
