Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, United States.
Department of Biochemistry, Emory University, Atlanta, GA, United States.
Curr Opin Chem Biol. 2014 Feb;18:55-61. doi: 10.1016/j.cbpa.2013.12.017. Epub 2014 Jan 29.
Host immunity represents a complex array of factors that evolved to provide protection against potential pathogens. While many factors regulate host immunity, glycan binding proteins (GBPs) appear to play a fundamental role in orchestrating this process. In addition, GBPs also reside at the key interface between host and pathogen. While early studies sought to understand GBP glycan binding specificity, limitations in the availability of test glycans made it difficult to elucidate a detailed understanding of glycan recognition. Recent developments in glycan microarray technology revolutionized analysis of GBP glycan interactions with significant implications in understanding the role of GBPs in host immunity. In this review, we explore different glycan microarray formats with a focus on the impact of these arrays on understanding the binding specificity and function of GBPs involved in immunity.
宿主免疫代表了一系列复杂的因素，这些因素的进化是为了提供对潜在病原体的保护。虽然许多因素调节宿主免疫，但糖结合蛋白（GBP）似乎在协调这一过程中起着至关重要的作用。此外，GBP 还存在于宿主和病原体的关键界面上。虽然早期的研究旨在了解 GBP 的糖结合特异性，但由于测试糖的可用性有限，很难详细了解糖的识别。糖微阵列技术的最新进展彻底改变了 GBP 糖相互作用的分析，这对理解 GBPs 在宿主免疫中的作用具有重要意义。在这篇综述中，我们探讨了不同的糖微阵列格式，重点讨论了这些阵列对理解参与免疫的 GBP 的结合特异性和功能的影响。
