Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, 44000, Pakistan.
Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
BMC Plant Biol. 2017 Oct 27;17(1):174. doi: 10.1186/s12870-017-1112-5.
P- type calcium ATPases (ACAs-auto inhibited calcium ATPases and ECAs-endoplasmic reticulum calcium ATPases) belong to the P- type ATPase family of active membrane transporters and are significantly involved in maintaining accurate levels of Ca, Mn and Zn in the cytosol as well as playing a very important role in stress signaling, stomatal opening and closing and pollen tube growth. Here we report the identification and possible role of some of these ATPases from wheat.
In this study, ACA and ECA sequences of six species (belonging to Poaceae) were retrieved from different databases and a phylogenetic tree was constructed. A high degree of evolutionary relatedness was observed among P sequences characterized in this study. Members of the respective groups from different plant species were observed to fall under the same clade. This pattern highlights the common ancestry of P type calcium ATPases. Furthermore, qRT-PCR was used to analyse the expression of selected ACAs and ECAs from Triticum aestivum (wheat) under calcium toxicity and calcium deficiency. The data indicated that expression of ECAs is enhanced under calcium stress, suggesting possible roles of these ATPases in calcium homeostasis in wheat. Similarly, the expression of ACAs was significantly different in plants grown under calcium stress as compared to plants grown under control conditions. This gives clues to the role of ACAs in signal transduction during calcium stress in wheat.
Here we concluded that wheat genome consists of nine P and three P -type calcium ATPases. Moreover, gene loss events in wheat ancestors lead to the loss of a particular homoeolog of a gene in wheat. To elaborate the role of these wheat ATPases, qRT-PCR was performed. The results indicated that when plants are exposed to calcium stress, both P and P gene expression get enhanced. This further gives clues about the possible role of these ATPases in wheat in calcium management. These findings can be useful in future for genetic manipulations as well as in wheat genome annotation process.
P 型钙 ATP 酶（ACAs-自动抑制钙 ATP 酶和 ECAs-内质网钙 ATP 酶）属于 P 型 ATP 酶家族的主动膜转运体，在维持细胞质中 Ca、Mn 和 Zn 的准确水平方面起着重要作用，同时在应激信号转导、气孔开闭和花粉管生长中也起着非常重要的作用。在这里，我们报告了从小麦中鉴定和可能发挥作用的一些 ATP 酶。
在这项研究中，从不同数据库中检索了六种物种（属于禾本科）的 ACA 和 ECA 序列，并构建了系统发育树。在所研究的 P 序列中观察到高度的进化相关性。不同植物物种的相应组的成员被观察到属于同一分支。这种模式突出了 P 型钙 ATP 酶的共同起源。此外，还使用 qRT-PCR 分析了小麦（Triticum aestivum）中选定的 ACAs 和 ECAs 在钙毒性和钙缺乏下的表达。数据表明，ECAs 的表达在钙胁迫下增强，这表明这些 ATP 酶可能在小麦的钙稳态中发挥作用。同样，与在对照条件下生长的植物相比，在钙胁迫下生长的植物中 ACAs 的表达有显著差异。这为 ACAs 在小麦钙胁迫信号转导中的作用提供了线索。
在这里，我们得出结论，小麦基因组包含九个 P 和三个 P-型钙 ATP 酶。此外，小麦祖先中的基因丢失事件导致了小麦中特定同源基因的丢失。为了阐述这些小麦 ATP 酶的作用，进行了 qRT-PCR。结果表明，当植物暴露于钙胁迫时，P 和 P 基因的表达都增强。这进一步说明了这些 ATP 酶在小麦钙管理中的可能作用。这些发现对未来的遗传操作以及小麦基因组注释过程都可能有用。
