Selim M I, Tsuei M H
Department of Preventive Medicine and Environmental Health, College of Medicine, University of Iowa, Iowa City 52242.
Am Ind Hyg Assoc J. 1993 Apr;54(4):135-41. doi: 10.1080/15298669391354478.
A new method for the extraction of aflatoxins from small quantities of airborne dust, using supercritical carbon dioxide, is described. The efficacy of this method is compared with a previously used liquid extraction procedure, using naturally contaminated grain dust samples. Supercritical fluid extraction (SFE) provides faster, more sensitive, and more selective extraction, compared with the liquid extraction method. The optimized SFE conditions for 0.1-1.2 g of dust in a 2.5 mL extraction chamber are: 2000 psi, 40 degrees C, 15 mL of liquid CO2, static extraction for 15 min, and 250 microL of acetonitrile added to sample in the extraction chamber. A detection limit of 1 ng aflatoxin B1 per sample is obtained using off-line HPLC analysis with UV detection. Lower detection limits are obtained using on-line SFE and HPLC analysis with fluorescence detection.
本文描述了一种使用超临界二氧化碳从少量空气尘埃中提取黄曲霉毒素的新方法。使用天然污染的谷物粉尘样本，将该方法的效果与先前使用的液体提取程序进行了比较。与液体提取方法相比，超临界流体萃取（SFE）提供了更快、更灵敏和更具选择性的提取。在2.5 mL萃取室中对0.1 - 1.2 g粉尘进行超临界流体萃取的优化条件为：2000 psi、40℃、15 mL液态二氧化碳、静态萃取15分钟，并向萃取室中的样品中加入250 μL乙腈。使用带有紫外检测的离线高效液相色谱分析，每个样品获得的黄曲霉毒素B1检测限为1 ng。使用在线超临界流体萃取和带有荧光检测的高效液相色谱分析可获得更低的检测限。
