Marmara University, Engineering Faculty, Bioengineering Department, Goztepe Campus, Istanbul, Turkey.
Can J Microbiol. 2011 Apr;57(4):295-302. doi: 10.1139/w11-009.
A gram-negative, moderately halophilic bacterium was isolated from Çamaltı Saltern area, located in the Aegean Region of Turkey. Analysis of its 16S rRNA gene sequence and physiological characteristics showed that this strain belonged to the genus Halomonas ; hence, it was designated as Halomonas sp. strain AAD12. The isolate tolerated up to 800 mg⋅L(-1) phenol; however, at elevated concentrations, phenol severely retarded cell growth. The increase in lag phase with increasing phenol concentrations indicated that the microorganism was undergoing serious adaptative changes. To understand the physiological responses of Halomonas sp. strain AAD12 to phenol, a 2-dimensional electrophoresis approach combined with mass spectrometric analysis was used. This approach showed that the expression of 14 protein spots were altered as phenol concentration increased from 200 to 800 mg⋅L(-1). Among the identified proteins were those involved in protein biosynthesis, energy, transport, and stress metabolism. So far, this is the first study on phenolic adaptation of a gram-negative, moderately halophilic bacteria using proteomic tools. The results provided new insights for understanding the general mechanism used by moderately halophilic bacteria to tolerate phenol and suggested the potential for using these microorganisms in bioremediation.
从位于土耳其爱琴海地区的恰米尔盐田地区分离到一株革兰氏阴性、中度嗜盐细菌。对其 16S rRNA 基因序列和生理特性的分析表明，该菌株属于盐单胞菌属；因此，将其命名为盐单胞菌 AAD12 株。该分离物可耐受高达 800mg⋅L(-1)的苯酚；然而，在较高浓度下，苯酚严重抑制细胞生长。随着苯酚浓度的增加，滞后期的增加表明微生物正在经历严重的适应性变化。为了了解盐单胞菌 AAD12 株对苯酚的生理反应，采用了二维电泳方法结合质谱分析。该方法表明，随着苯酚浓度从 200mg⋅L(-1)增加到 800mg⋅L(-1)，有 14 个蛋白点的表达发生了改变。鉴定出的蛋白包括参与蛋白质生物合成、能量、运输和应激代谢的蛋白。到目前为止，这是使用蛋白质组学工具研究革兰氏阴性、中度嗜盐细菌对苯酚适应的首次研究。研究结果为理解中度嗜盐细菌耐受苯酚的一般机制提供了新的见解，并表明这些微生物在生物修复中的应用潜力。
