Knuppe Molecular Urology Laboratory, Department of Urology, University of California, San Francisco, CA 94143-0738, USA.
Urology. 2011 Jun;77(6):1509.e1-8. doi: 10.1016/j.urology.2010.12.076. Epub 2011 Apr 13.
To investigate whether adipose-derived matrix seeded with adipose-derived stem cells (ADSC) can facilitate the repair of injured cavernous nerves (CNs).
Human and rat adipose tissues were decellularized and fabricated into various forms, including adipose tissue-derived acellular matrix thread (ADMT). ADMT seeded with ADSC were transplanted into subcutaneous space and examined for signs of inflammation. ADSC-seeded ADMTs were then used to repair CN injury in rats, followed by assessment of histology and erectile function.
Adipose tissue can be fabricated into acellular matrices of various shapes and sizes, including threads and sheets. Seeding of ADMT occurred rapidly: within 24 hours, 55% of the surface was covered with ADSC and within 1 week, 90% was covered. Transplantation of the seeded ADMT into the subcutaneous space of an allogenic host showed no signs of inflammatory reaction. At 3 months after grafting into CN injury rats, approximately twice as many cells were found on seeded ADMT as on unseeded ADMT. The seeded ADMT also had various degrees of S100 and neuronal nitric oxide synthase expression, suggesting CN axonal ingrowth. Rats grafted with seeded ADMT overall had the best erectile function recovery when compared with those grafted with unseeded ADMT and those ungrafted. However, as a result of large variations, the differences did not reach statistic significance (P = .07).
Grafting of ADSC-seeded matrix resulted in a substantial recovery of erectile function and improvement of histology. However, further refinement of the matrix architecture is needed to improve the success rate.
研究脂肪来源基质（ADM）负载脂肪来源干细胞（ADSC）是否能促进受损海绵体神经（CN）的修复。
对人及大鼠脂肪组织进行脱细胞处理，制成各种形式的材料，包括脂肪组织衍生去细胞基质线（ADMT）。将 ADSC 负载到 ADMT 上，移植到皮下空间，观察炎症迹象。然后，将负载 ADSC 的 ADMT 用于修复大鼠的 CN 损伤，随后评估组织学和勃起功能。
脂肪组织可制成各种形状和大小的无细胞基质，包括线和片。ADMT 的种子细胞快速定植：24 小时内，有 55%的表面被 ADSC 覆盖，1 周内，90%的表面被覆盖。将负载 ADSC 的 ADMT 移植到同种异体宿主的皮下空间中，没有炎症反应的迹象。在移植到 CN 损伤大鼠 3 个月后，负载 ADSC 的 ADMT 上发现的细胞数量大约是未负载 ADSC 的 ADMT 的两倍。负载 ADSC 的 ADMT 还具有不同程度的 S100 和神经元型一氧化氮合酶表达，提示 CN 轴突生长。与未负载 ADSC 和未移植的大鼠相比，负载 ADSC 的大鼠的勃起功能整体恢复最好。然而，由于存在较大的差异，这些差异没有达到统计学意义（P =.07）。
ADSC 负载基质的移植导致勃起功能有显著恢复，组织学得到改善。然而，需要进一步改进基质结构，以提高成功率。
