Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Vet Rec. 2011 Feb 12;168(6):160. doi: 10.1136/vr.c6479. Epub 2011 Feb 7.
Serum samples from 1120 layers from 56 flocks and 400 pullets from 20 flocks were tested by an indirect sandwich ELISA to investigate the prevalence of antibodies to Histomonas meleagridis in chickens kept in alternative husbandry systems. The overall prevalence of antibodies to H meleagridis in layers was 37.3 per cent, and positive birds were identified in 50 flocks. This was significantly higher than in pullets, where only 8.3 per cent of the birds tested positive. Optical density (OD) values obtained from pullet sera were much lower than the OD values from layers; however, positive birds were detected in half of the pullet flocks. In particular, all birds from an organic pullet flock were found to be positive, with high OD values. Overall, the highest prevalence of positive sera was obtained from birds kept in free-range flocks. Attempts to reisolate live histomonads from birds in 18 layer flocks were unsuccessful.
采用间接夹心 ELISA 法检测了来自 56 个鸡群的 1120 个血清样本和来自 20 个鸡群的 400 只育成母鸡，以调查在替代养殖系统中饲养的鸡对火鸡组织滴虫的抗体流行情况。鸡群中组织滴虫抗体的总体阳性率为 37.3%，在 50 个鸡群中发现了阳性鸡。这明显高于育成母鸡，其中只有 8.3%的鸡呈阳性。从育成母鸡血清中获得的光密度 (OD) 值远低于鸡群的 OD 值；然而，在一半的育成母鸡鸡群中检测到了阳性鸡。特别是，从一个有机育成母鸡鸡群中的所有鸡都被发现呈阳性，OD 值较高。总体而言，从自由放养鸡群中获得的阳性血清阳性率最高。从 18 个鸡群中尝试再次分离活的组织滴虫也未成功。
