Department of Obstetrics and Gynecology, Dokuz Eylul University Medical School, Dokuz Eylul University Hospital, Izmir, Turkey.
Fertil Steril. 2011 Jun;95(7):2274-8. doi: 10.1016/j.fertnstert.2011.03.045. Epub 2011 Apr 15.
To evaluate the association between follicular fluid levels of propeptide and mature forms of growth differentiation factor (GDF) 9 and bone morphogenetic protein (BMP) 15 with subsequent oocyte and embryo quality.
Prospective clinical study.
University hospital.
PATIENT(S): Eighty-one infertile patients who underwent in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI).
INTERVENTION(S): The expression levels of the propeptide and mature forms of follicular fluid GDF9 and BMP15 were determined by western blot analysis. The levels of follicular fluid hormones (FSH, E2, and P) were measured with automated chemiluminescent enzyme immunoassays.
MAIN OUTCOME MEASURE(S): The relationships between the levels of GDF9 and BMP15, hormones, oocyte maturation, and embryo quality.
RESULT(S): Mature GDF9 levels were significantly correlated with the nuclear maturation of oocytes. The mean mature GDF9 level was 4.87±0.60 in the high-embryo-quality group and 1.45±0.81 in the low-embryo-quality group. There were no statistically significant differences in embryo quality among the patients regarding propeptide GDF9 and BMP15 expression status. There was a negative correlation between follicular fluid levels of P and the mature form of GDF9.
CONCLUSION(S): Higher mature GDF9 levels in the follicular fluid were significantly correlated with oocyte nuclear maturation and embryo quality.
评估卵泡液中生长分化因子 9（GDF9）和骨形态发生蛋白 15（BMP15）前肽和成熟形式与随后卵母细胞和胚胎质量的关系。
前瞻性临床研究。
大学医院。
81 名接受体外受精（IVF）/胞浆内单精子注射（ICSI）的不孕患者。
通过 Western blot 分析测定卵泡液中 GDF9 和 BMP15 的前肽和成熟形式的表达水平。用自动化化学发光酶免疫分析法测定卵泡液激素（FSH、E2 和 P）的水平。
GDF9 和 BMP15 水平、激素、卵母细胞成熟和胚胎质量之间的关系。
成熟 GDF9 水平与卵母细胞的核成熟显著相关。高胚胎质量组的平均成熟 GDF9 水平为 4.87±0.60，低胚胎质量组为 1.45±0.81。在考虑前肽 GDF9 和 BMP15 表达状态的情况下，患者的胚胎质量之间没有统计学差异。卵泡液中 P 水平与 GDF9 成熟形式之间存在负相关。
卵泡液中较高的成熟 GDF9 水平与卵母细胞核成熟和胚胎质量显著相关。
