开发一种用于古菌病毒 Sulfolobus turreted icosahedral virus (STIV) 的遗传系统。

Development of a genetic system for the archaeal virus Sulfolobus turreted icosahedral virus (STIV).

机构信息

Thermal Biology Institute, Montana State University, Bozeman, MT, USA.

出版信息

Virology. 2011 Jun 20;415(1):6-11. doi: 10.1016/j.virol.2011.03.023. Epub 2011 Apr 15.

DOI:10.1016/j.virol.2011.03.023

Abstract

Our understanding of archaeal viruses has been limited by the lack of genetic systems for examining viral function. We describe the construction of an infectious clone for the archaeal virus Sulfolobus turreted icosahedral virus (STIV). STIV was isolated from a high temperature (82°C) acidic (pH 2.2) hot spring in Yellowstone National Park and replicates in the archaeal model organism Sulfolobus solfataricus (Rice et al., 2004). While STIV is one of most studied archaeal viruses, little is known about its replication cycle. The development of an STIV infectious clone allows for directed gene disruptions and detailed genetic analysis of the virus. The utility of the STIV infectious clone was demonstrated by gene disruption of STIV open reading frame (ORF) B116 which resulted in crippled virus replication, while disruption of ORFs A197, C381 and B345 was lethal for virus replication.

摘要

我们对古菌病毒的了解受到缺乏用于检查病毒功能的遗传系统的限制。我们描述了古菌病毒 Sulfolobus 塔式二十面体病毒 (STIV) 的感染性克隆的构建。STIV 是从黄石国家公园高温（82°C）酸性（pH 2.2）温泉中分离出来的，在古菌模式生物 Sulfolobus solfataricus 中复制（Rice 等人，2004 年）。虽然 STIV 是研究最多的古菌病毒之一，但对其复制周期知之甚少。STIV 感染性克隆的开发允许对病毒进行定向基因敲除和详细的遗传分析。通过 STIV 开放阅读框 (ORF) B116 的基因敲除证明了 STIV 感染性克隆的实用性，这导致病毒复制受损，而 ORFs A197、C381 和 B345 的敲除对病毒复制是致命的。