South Texas Center for Emerging Infectious Diseases and Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249-1644, United States.
Cell Immunol. 2011;269(2):69-73. doi: 10.1016/j.cellimm.2011.03.023. Epub 2011 Mar 29.
The bacterial determinants of pulmonary Francisella induced inflammatory responses and their interaction with host components are not clearly defined. In this study, proteomic and immunoblot analyses showed presence of a cytoplasmic protein elongation factor Tu (EF-Tu) in the membrane fractions of virulent Francisella novicida, LVS and SchuS4, but not in an attenuated F. novicida mutant. EF-Tu was immunodominant in mice vaccinated and protected from virulent F. novicida. Moreover, recombinant EF-Tu induced macrophages to produce inflammatory cytokines in a TLR4 dependent manner. This study shows immune stimulatory properties of a cytoplasmic protein EF-Tu expressed on the membrane of virulent Francisella strains.
肺部弗朗西斯菌引起的炎症反应的细菌决定因素及其与宿主成分的相互作用尚不清楚。在这项研究中，蛋白质组学和免疫印迹分析显示，毒力弗朗西斯菌 novicida、LVS 和 SchuS4 的膜部分存在细胞质蛋白延伸因子 Tu（EF-Tu），但在减毒的 F. novicida 突变体中不存在。EF-Tu 在接种疫苗并免受毒力 F. novicida 侵害的小鼠中具有免疫优势。此外，重组 EF-Tu 以 TLR4 依赖的方式诱导巨噬细胞产生炎症细胞因子。这项研究表明，在毒力弗朗西斯菌菌株的膜上表达的细胞质蛋白 EF-Tu 具有免疫刺激特性。
