Butnaru Maria, Dimitriu Cristina-Daniela, Macocinschi Doina
Facultatea de Bioinginerie Medicală, Disciplina de Biologie celulară si moleculară,Universitatea de Medicină si Farmacie Gr.T. Popa Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2010 Oct-Dec;114(4):1246-53.
The polyurethanes are very used materials for medical devices. Their segmented block-copolymeric structures endows them a wide range of versatility in terms of tailoring their physical properties, and tissue compatibility.
A new polyether-urethane based material (PU-PTHF/MDI/HPC) was studied on bio-integration capacity in cardiovascular applications. In this respect, surface and bulk structure analysis, albumin and fibrinogen adsorption (in noncompetitive and competitive condition) and thrombogenicity have been evaluated. It was found that PU-PTHF/MDI/HPC surfaces had different roughness and bulk interconnected, well developed porous structure. As protein adsorption properties, preferential adsorption for albumin of material was observed.
Thus, 0.280 +/- 0.04 mg/cm2 and 0.260+0.01 mg/ cm2 of albumin adsorb on PU-PTHF/MDI/HPC in noncompetitive and competitive conditions, while fibrinogen adsorption was 0.044 +/- 0.02 mg/cm2 and 0.040 +/- 0.01 mg/cm2. The clot amount test experiment have shown that clot weight of control blood was 24.8 +/- 2.03 mg, the clot weight formed by polyurethane was 28.4 +/- 2.93 mg and the clot weight formed by thrombogenic positive control (collagen membrane) was 42.5 +/- 3.50 mg.
From our study we could conclude: 1--PU-PTHF/MDI/HPC polyurethane membranes have two-interface structure that could be advantageous for cavitary or tubular medical devices; 2--the protein adsorption results and clot formation test revealed a thromboresistent behavior of PU-PTHF/MDI/HPC, appropriate for cardiovascular applications.
聚氨酯是医疗器械中常用的材料。其嵌段共聚物结构使其在调整物理性能和组织相容性方面具有广泛的通用性。
研究了一种新型聚醚聚氨酯基材料（PU-PTHF/MDI/HPC）在心血管应用中的生物整合能力。在此方面，评估了表面和本体结构分析、白蛋白和纤维蛋白原吸附（在非竞争和竞争条件下）以及血栓形成性。发现PU-PTHF/MDI/HPC表面具有不同的粗糙度，且本体相互连通、结构良好的多孔结构。作为蛋白质吸附特性，观察到该材料对白蛋白有优先吸附作用。
因此，在非竞争和竞争条件下，分别有0.280±0.04 mg/cm²和0.260 + 0.01 mg/cm²的白蛋白吸附在PU-PTHF/MDI/HPC上，而纤维蛋白原吸附量分别为0.044±0.02 mg/cm²和0.040±0.01 mg/cm²。凝血量测试实验表明，对照血液的凝块重量为24.8±2.03 mg，聚氨酯形成的凝块重量为28.4±2.93 mg，血栓形成阳性对照（胶原膜）形成的凝块重量为42.5±3.50 mg。
从我们的研究中可以得出：1——PU-PTHF/MDI/HPC聚氨酯膜具有双界面结构，这对腔状或管状医疗器械可能有利；2——蛋白质吸附结果和凝血形成测试表明PU-PTHF/MDI/HPC具有抗血栓行为，适用于心血管应用。
