National Research Laboratory for Mitochondrial Signaling, Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University 633-165 Gaegeum-Dong, Busanjin-Gu, Busan 613-735, Korea.
Expert Rev Proteomics. 2011 Apr;8(2):241-61. doi: 10.1586/epr.11.8.
Cardiac ischemia and ischemia-reperfusion (I/R) injury are major contributors to morbidity and mortality worldwide. Pathological mechanisms of I/R and the physiological mechanisms of ischemic preconditioning (IPC), which is an effective cardiac protective response, have been widely investigated in the last decade to search for means to prevent or treat this disease. Proteomics is a powerful analytical tool that has provided important information to identify target proteins and understand the underlying mechanisms of I/R and IPC. Here, we review the application of proteomics to I/R injury and IPC to discover target proteins. We analyze the functional meaning of the accumulated data on hundreds of proteins using various bioinformatics applications. In addition, we review exercise-induced proteomic alterations in the heart to understand the potential cardioprotective role of exercise against I/R injury. Further developments in the proteomic field that target specialized proteins will yield new insights for optimizing therapeutic targets and developing a wide range of therapeutic agents against ischemic heart disease.
心肌缺血和缺血再灌注（I/R）损伤是全球发病率和死亡率的主要原因。在过去的十年中，广泛研究了 I/R 的病理机制和缺血预处理（IPC）的生理机制，这是一种有效的心脏保护反应，以寻找预防或治疗这种疾病的方法。蛋白质组学是一种强大的分析工具，为鉴定靶蛋白和理解 I/R 和 IPC 的潜在机制提供了重要信息。在这里，我们回顾了蛋白质组学在 I/R 损伤和 IPC 中的应用，以发现靶蛋白。我们使用各种生物信息学应用程序分析了数百种蛋白质的累积数据的功能意义。此外，我们还回顾了运动引起的心脏蛋白质组变化，以了解运动对 I/R 损伤的潜在心脏保护作用。针对特定蛋白质的蛋白质组学领域的进一步发展将为优化治疗靶点和开发针对缺血性心脏病的广泛治疗药物提供新的见解。
