一种新型强效病毒诱导剂在人细胞培养物中诱导干扰素的产生。

Production of interferon in human cell cultures by a new, potent viral inducer.

作者信息

Jameson P, Grossberg S E

出版信息

Adv Exp Med Biol. 1978;110:37-53. doi: 10.1007/978-1-4615-9080-4_4.

Abstract

A new discovered double-stranded RNA inducer of interferon, bluetongue virus (BTV), stimulates the production of large amounts of interferon in animals as well as in many types of mammalian cell cultures, including human leukocytes, and continuous cell lines. The exceptional pH lability of BTV and its lack of pathogenicity for man further recommend its use as an interferon inducer. Among several human cell lines tested, the most efficient producer of interferon was a continuous cell line designated HT-1376, derived from a bladder carcinoma. With infectious BTV as the inducer, the HT-1376 line produced more interferon per cell than did leukocytes; interferon yields ranged from 10,000 to 60,000 units per ml of crude, unconcentrated supernatant fluid. Noninfectious BTV, inactivated by ultraviolet irradiation, was as effective as infectious virions. The interferon produced in HT-1376 cells has physicochemical and antigenic properties resembling those of fibroblast interferon produced in diploid cells.

摘要

一种新发现的干扰素双链RNA诱导剂——蓝舌病毒（BTV），能刺激动物以及多种哺乳动物细胞培养物（包括人类白细胞和连续细胞系）产生大量干扰素。BTV特殊的pH不稳定性及其对人类无致病性，进一步表明它可作为干扰素诱导剂。在测试的几种人类细胞系中，最有效的干扰素产生细胞系是一种名为HT - 1376的连续细胞系，它源自膀胱癌。以感染性BTV作为诱导剂时，HT - 1376细胞系每个细胞产生的干扰素比白细胞更多；干扰素产量在每毫升未浓缩的粗制上清液中为10,000至60,000单位。经紫外线照射灭活的非感染性BTV与感染性病毒粒子一样有效。HT - 1376细胞产生的干扰素具有与二倍体细胞产生的成纤维细胞干扰素相似的物理化学和抗原特性。