Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600 036, India.
Colloids Surf B Biointerfaces. 2011 Aug 1;86(1):146-53. doi: 10.1016/j.colsurfb.2011.03.033. Epub 2011 Apr 1.
Biocompatibility of medical grade polyurethane coated with polyaniline (PANi) and polyaniline-silver nanoparticle composite (PANi-AgNp) is reported here. These modified films showed 23 and 18% of 3T3 L1 cell death when compared to 41% with virgin polyurethane (PU) after 48h of incubation, respectively. All the surfaces elucidated inflammatory response in the form of enhanced expressions of the proinflammatory cytokines genes, TNF-α and IL-6. But these values were less (by 20%) on modified films than on the bare PU. Attachment of Pseudomonas and Bacillus were markedly less on PANi-AgNp coated surface (by 90.6 and 50.5%, respectively) when compared to the uncoated PU. As the CFU counts decreases on the nanoparticle coated PU, the adsorbed carbohydrate as well as protein content on to the surface of polymer decreases accordingly, indicating less attachment. A 20% reduction in the thickness of biofilm was observed in PANi-AgNp coated PU surface. A very strong positive correlation is observed between the contact angles of the polymers and the various biological parameters (namely colony forming units, protein, carbohydrate, cell death and inflammatory response), indicating hydrophilic surfaces prevent bacterial biofilm as well as are compatible to cells when compared to hydrophobic surfaces. Coating PU with PANi and PANi+AgNp renders the surface conductive, suggesting potential application in electrochemical biosensors. In addition, these modifications make the surface more biocompatible than the original PU.
本文报道了经聚苯胺（PANI）和聚苯胺-银纳米粒子复合材料（PANi-AgNp）修饰的医用级聚氨酯的生物相容性。与原始的聚氨酯（PU）相比，这些修饰膜在孵育 48 小时后，分别有 23%和 18%的 3T3 L1 细胞死亡，而原始的聚氨酯（PU）则有 41%的细胞死亡。所有的表面都以增强促炎细胞因子基因（TNF-α 和 IL-6）的表达形式表现出炎症反应。但是，这些值在修饰膜上比在裸 PU 上要低（低 20%）。与未涂层的 PU 相比，PANI-AgNp 涂层表面上的 Pseudomonas 和 Bacillus 的附着明显减少（分别减少了 90.6%和 50.5%）。由于纳米颗粒涂层 PU 上的 CFU 计数减少，吸附在聚合物表面上的碳水化合物和蛋白质含量相应减少，表明附着减少。在 PANi-AgNp 涂层 PU 表面观察到生物膜厚度减少 20%。聚合物的接触角与各种生物学参数（即菌落形成单位、蛋白质、碳水化合物、细胞死亡和炎症反应）之间存在很强的正相关关系，这表明与疏水性表面相比，亲水性表面可以防止细菌生物膜的形成，并与细胞相容。用 PANi 和 PANi+AgNp 涂覆 PU 会使表面具有导电性，这表明其在电化学生物传感器中有潜在的应用。此外，这些修饰使表面比原始的 PU 更具生物相容性。
