Department of Chemical Engineering, School of Engineering, University of Santiago de Compostela, Santiago de Compostela, Spain.
Biodegradation. 2011 Jun;22(3):539-50. doi: 10.1007/s10532-010-9426-0. Epub 2010 Oct 24.
Pharmaceuticals are an important group of emerging pollutants with increasing interest due to their rising consumption and the evidence for ecotoxicological effects associated to trace amounts in aquatic environments. In this paper, we assessed the potential degradation of a series of pharmaceuticals: antibiotics (sulfamethoxazole), antidepressives (citalopram hydrobromide and fluoxetine hydrochloride), antiepileptics (carbamazepine), anti-inflammatory drugs (diclofenac and naproxen) and estrogen hormones (estrone, 17β-estradiol, 17α-ethinylestradiol) by means of a versatile peroxidase (VP) from the ligninolytic fungus Bjerkandera adusta. The effects of the reaction conditions: VP activity, organic acid concentration and H(2)O(2) addition rate, on the kinetics of the VP based oxidation system were evaluated. Diclofenac and estrogens were completely degraded after only 5-25 min even with a very low VP activity (10 U l(-1)). High degradation percentages (80%) were achieved for sulfamethoxazole and naproxen. Low or undetectable removal yields were observed for citalopram (up to 18%), fluoxetine (lower than 10%) and carbamazepine (not degraded).
药品是新兴污染物的重要组成部分，由于其消耗量不断增加，以及在水生环境中痕量存在时会产生生态毒理学效应的证据，人们对其越来越感兴趣。在本文中，我们评估了一系列药品（抗生素（磺胺甲恶唑）、抗抑郁药（氢溴酸西酞普兰和盐酸氟西汀）、抗癫痫药（卡马西平）、消炎药（双氯芬酸和萘普生）和雌激素激素（雌酮、17β-雌二醇、17α-乙炔基雌二醇））的潜在降解能力，方法是使用木腐真菌 Bjerkandera adusta 中的一种多功能过氧化物酶（VP）。评估了反应条件（VP 活性、有机酸浓度和 H(2)O(2)添加速率）对 VP 基氧化体系动力学的影响。即使 VP 活性（10 U l(-1)）非常低，双氯芬酸和雌激素也仅在 5-25 分钟内完全降解。磺胺甲恶唑和萘普生的降解率很高（80%）。西酞普兰（高达 18%）、氟西汀（低于 10%）和卡马西平（未降解）的去除率低或无法检测到。
