Mícková V
Státní veterinární ústav, Ostrava.
Cesk Epidemiol Mikrobiol Imunol. 1990 Jul;39(4):222-7.
The paper deals with the incidence of Yersiniae in pork and beef, focused in particular on the prevalence of Yersinia enterocolitica, serovar 3, biovar 4. The author uses for detection the method of cold shock, alkalization of the sample, taking advantage of the tolerance of Yersinias of diluted alkalis and suppressing the contaminating microflora, and the the selective medium CIN agar with a locally manufactured supplement. Cultivation was either direct or after propagation in phosphate buffer pH 7.6. The authors isolated 84 strains of Yersinias, i.e. 60% of the total number of examined specimens. Four strains were Yersinia enterocolitica, serovar 03, biovar 4 from pork by direct cultivation. Twenty strains did not agglutinate with commercial serum O-Yersinia IMUNA; they were included in the group "Yersinia enterocolitica other biovars" and 60 strains were "Environmental Yersinia isolates". The author proved a seasonal incidence of Yersinie enterocolitica, serovar 03, biovar 4. The applied method of detection and the the selective medium CIN agar with supplement proved suitable. It is important to respect hygienic provisions during processing in the slaughterhouse with regard to the high incidence of environmental Yersinias and the possible incidence of pathogenic serovars of Yersinias.
本文探讨猪肉和牛肉中耶尔森氏菌的发生率，尤其关注小肠结肠炎耶尔森氏菌3型、生物变种4型的流行情况。作者采用冷休克法、样品碱化法进行检测，利用耶尔森氏菌对稀释碱的耐受性并抑制污染微生物群，以及使用添加了本地自制添加剂的选择性培养基CIN琼脂。培养方式为直接培养或在pH 7.6的磷酸盐缓冲液中传代培养后进行培养。作者分离出84株耶尔森氏菌，占检测标本总数的60%。通过直接培养从猪肉中分离出4株小肠结肠炎耶尔森氏菌03型、生物变种4型。20株与商业O型耶尔森氏菌免疫血清不发生凝集反应；它们被归入“其他生物变种的小肠结肠炎耶尔森氏菌”组，60株为“环境耶尔森氏菌分离株”。作者证实了小肠结肠炎耶尔森氏菌03型、生物变种4型的季节性发生率。所应用的检测方法以及添加添加剂的选择性培养基CIN琼脂被证明是合适的。鉴于环境耶尔森氏菌的高发生率以及耶尔森氏菌致病血清型的可能发生率，在屠宰场加工过程中遵守卫生规定非常重要。
