Laukkanen-Ninios Riikka, Fredriksson-Ahomaa Maria, Maijala Riitta, Korkeala Hannu
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, P.O. Box 66, FIN-00014, University of Helsinki, Finland.
Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, P.O. Box 66, FIN-00014, University of Helsinki, Finland.
Food Microbiol. 2014 Oct;43:50-2. doi: 10.1016/j.fm.2014.04.016. Epub 2014 May 4.
Samples from pork cuts for minced meat and cheeks from processing plants and a slaughterhouse, and modified atmosphere (MA) packaged pork from retail were studied to estimate the prevalence of pathogenic, i.e. virulence plasmid bearing, Yersinia enterocolitica and Yersinia pseudotuberculosis in pork, as well as to quantify pathogenic Y. enterocolitica in pork cuts. Pathogenic (virF-positive) Y. enterocolitica was isolated from 17 pig cheeks (23%) but not from any of the MA-packaged 54 retail pork samples and only from one of the 155 pork cut (0.6%). Most (16/17) of the cheek samples were contaminated with pathogenic Y. enterocolitica 4/O:3 and one with bioserotype 2/O:9. No Y. pseudotuberculosis was isolated. The prevalence of pathogenic Y. enterocolitica was clearly higher (39%) in 155 pork cuts when studied with nested PCR targeting yadA on the virulence plasmid pYV although the contamination level was low varying between 0.1 and 1.6 MPN/g. Raw pork cuts and especially pig cheeks may serve as possible sources for yersiniosis caused by pathogenic Y. enterocolitica.
对来自加工厂和屠宰场的用于制作碎肉的猪肉切块以及猪脸颊肉样本，以及来自零售渠道的气调包装猪肉进行了研究，以估计猪肉中致病性（即携带毒力质粒）小肠结肠炎耶尔森菌和假结核耶尔森菌的流行率，并对猪肉切块中的致病性小肠结肠炎耶尔森菌进行定量。从17个猪脸颊肉样本（23%）中分离出了致病性（virF阳性）小肠结肠炎耶尔森菌，但在54个气调包装的零售猪肉样本中均未分离到，在155个猪肉切块样本中仅从1个样本（0.6%）中分离到。大多数（16/17）脸颊肉样本被致病性小肠结肠炎耶尔森菌4/O:3污染，1个样本被生物血清型2/O:9污染。未分离到假结核耶尔森菌。当用针对毒力质粒pYV上yadA的巢式PCR进行研究时，155个猪肉切块中致病性小肠结肠炎耶尔森菌的流行率明显更高（39%），尽管污染水平较低，在0.1至1.6 MPN/g之间变化。生猪肉切块，尤其是猪脸颊肉可能是致病性小肠结肠炎耶尔森菌引起耶尔森菌病的可能来源。
