Hunsperger Elizabeth, Beltran Manuela, Acosta Luz Nereida, Jordan-Munoz Jorge, Torres Jomil, Luce Richard, Tomashek Kay M
Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Dengue Branch, San Juan, Puerto Rico.
Clin Vaccine Immunol. 2011 Jun;18(6):978-83. doi: 10.1128/CVI.00040-11. Epub 2011 Apr 20.
A laboratory testing algorithm was evaluated to confirm West Nile virus (WNV) infection in human serum following the introduction of the virus in Puerto Rico in 2007. This testing algorithm used two standard diagnostic assays, the IgM antibody capture enzyme-linked immunosorbent assay (MAC ELISA) and real-time reverse transcriptase PCR (RT-PCR), along with two nonconventional assays, the nonstructural protein 1 (NS1) ELISA and a 90%-plaque-reduction neutralization test (PRNT(90)) with IgG depletion for dengue virus (DENV) and WNV. A total of 2,321 serum samples from suspected WNV human cases were submitted for testing. Approximately one-third (867, 37%) were cross-reactive for DENV and WNV by MAC ELISA and had negative RT-PCR results for both viruses. Of a subset of 43 samples tested, 31 (72%) of these cases were identified as positive for DENV in the PRNT(90) with IgG depletion and 8 (19%) were positive in the DENV NS1 antigen ELISA. These two assays combined differentiated 36 (84%) of the samples that could not be diagnosed using the standard diagnostic testing methods.
2007年西尼罗河病毒（WNV）传入波多黎各后，对一种用于确认人血清中WNV感染的实验室检测算法进行了评估。该检测算法使用了两种标准诊断检测方法，即IgM抗体捕获酶联免疫吸附测定（MAC ELISA）和实时逆转录聚合酶链反应（RT-PCR），以及两种非常规检测方法，即非结构蛋白1（NS1）ELISA和针对登革病毒（DENV）和WNV的经IgG去除的90%蚀斑减少中和试验（PRNT(90)）。总共提交了2321份来自疑似WNV人类病例的血清样本进行检测。通过MAC ELISA，约三分之一（867份，37%）的样本对DENV和WNV呈交叉反应，且两种病毒的RT-PCR结果均为阴性。在检测的43份样本子集中，这些病例中有31份（72%）在经IgG去除的PRNT(90)中被鉴定为DENV阳性，8份（19%）在DENV NS1抗原ELISA中呈阳性。这两种检测方法相结合，区分出了36份（84%）无法使用标准诊断检测方法进行诊断的样本。
