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一种用于分离和研究趋磁细菌的新方法。

A novel method for the isolation and study of a magnetotactic bacterium.

作者信息

Moench T T, Konetzka W A

出版信息

Arch Microbiol. 1978 Nov 13;119(2):203-12. doi: 10.1007/BF00964274.

DOI:10.1007/BF00964274
PMID:215094
Abstract

The magnetococcus, a magnetotactic bacterium, has been grown in a complex simulated natural environment. Sufficiently pure samples of cells were obtained magnetically making axenic cultures unnecessary for many purposes. The magnetococcus is a Gram-negative coccus, 1.6 micron in diameter and readily distinguished by highly refractile inclusions and its magnetotactic behavior. This organism is actively motile by means of two bundles of flagella. Electron dense ferromagnetic inclusions were localized between the flagellar bundles. Collections of magnetococci were morphologically homogeneous and negligibly contaminated by extraneous bacteria. DNA extracted from pooled collections of cells was homogeneous by analytical CsC1 centrifugation. The guanine-cytosine content was 61.7%. Total iron by percent cellular dry weight was 3.8%. Comparisons with a previously described magnetotactic marine coccus were made.

摘要

趋磁球菌是一种趋磁细菌,已在复杂的模拟自然环境中培养。通过磁性方法获得了足够纯净的细胞样本,使得在许多情况下无需进行无菌培养。趋磁球菌是革兰氏阴性球菌,直径为1.6微米,很容易通过高度折射的内含物及其趋磁行为来区分。这种生物体通过两束鞭毛进行活跃运动。电子致密的铁磁内含物位于鞭毛束之间。趋磁球菌的聚集体在形态上是均匀的,外来细菌的污染可忽略不计。通过分析性CsC1离心法,从合并的细胞聚集体中提取的DNA是均匀的。鸟嘌呤 - 胞嘧啶含量为61.7%。细胞干重中铁的总含量为3.8%。并与先前描述的趋磁海洋球菌进行了比较。

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1
A novel method for the isolation and study of a magnetotactic bacterium.一种用于分离和研究趋磁细菌的新方法。
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2
Magnetotactic bacteria.趋磁细菌
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2
Genome-Based Metabolic Reconstruction of a Novel Uncultivated Freshwater Magnetotactic coccus ". Magnetaquicoccus inordinatus" UR-1, and Proposal of a Candidate Family ". Magnetaquicoccaceae".基于基因组的新型未培养淡水趋磁球菌“不规则趋磁水球菌”UR-1的代谢重建及候选科“趋磁水球菌科”的提议
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Single-cell determination of iron content in magnetotactic bacteria: implications for the iron biogeochemical cycle.

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