van Doorn Nienke L, Wilson Andrew S, Willerslev Eske, Gilbert M Thomas P
Department of Archaeological Science, University of Bradford, Richmond Road, BD1 5DD Bradford, UK.
J Forensic Sci. 2011 May;56(3):720-5. doi: 10.1111/j.1556-4029.2010.01684.x. Epub 2011 Feb 3.
The susceptibility of RNA to enzymatic degradation has been considered as a tool to estimate time-since-death in forensic samples, and it has previously been demonstrated that the choice of tissue is an important factor. In this study we have extracted RNA from decaying bone and bone marrow under the hypothesis that the delayed onset of putrefaction may render them a useful source in this context. In a preliminary study, total RNA was extracted from bone and bone marrow that had been sampled from six skeletally mature rabbits at time points between zero and 31 days after death. The levels of three specific RNA transcripts could be quantified using real-time polymerase chain reaction. Bioanalyzer results show rRNA bands in bone marrow samples up to 21 days postmortem. We hereby propose bone marrow as a potential source for postmortem RNA in forensic studies.
RNA对酶促降解的敏感性已被视为法医样本中估计死亡时间的一种工具，并且此前已经证明组织的选择是一个重要因素。在本研究中，我们在腐烂延迟开始可能使它们在这种情况下成为有用来源的假设下，从腐烂的骨骼和骨髓中提取了RNA。在一项初步研究中，从6只骨骼成熟的兔子在死后0至31天的时间点采集的骨骼和骨髓中提取了总RNA。可以使用实时聚合酶链反应对三种特定RNA转录本的水平进行定量。生物分析仪结果显示，死后21天内骨髓样本中存在rRNA条带。我们在此提出，骨髓可作为法医研究中死后RNA的潜在来源。
