Biomicrofluidics. 2011 Mar 30;5(1):13414. doi: 10.1063/1.3571477.
This study presents a novel approach to decant supernatant on a centrifugal platform. By manipulating the centrifugally induced pressure and the elastic deformation of the plastic lids in the decanting chamber, fixed amounts of the supernatant can be decanted into the detection chamber at lower rotational speeds. The experimental results showed that decanted volume is affected by the volume of deformation and the operating parameters. Factors that influence the decanting ratio are also discussed. This approach has the advantages of simple design and low manufacturing cost; further, it has no need of surface modification. It has been applied to on-disk separation of plasma from whole blood, and the results showed good stability and repeatability.
本研究提出了一种在离心平台上倾析上清液的新方法。通过操纵离心诱导压力和倾析室中塑料盖的弹性变形，可以在较低的转速下将固定量的上清液倾析到检测室中。实验结果表明，倾析体积受变形体积和操作参数的影响。还讨论了影响倾析比的因素。该方法具有设计简单、制造成本低的优点；此外，它不需要表面改性。它已应用于全血中血浆的盘上分离，结果表明具有良好的稳定性和重现性。
