Steigert J, Brenner T, Grumann M, Riegger L, Lutz S, Zengerle R, Ducrée J
Laboratory for MEMS Applications, Department for Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 106, 79110, Freiburg, Germany.
Biomed Microdevices. 2007 Oct;9(5):675-9. doi: 10.1007/s10544-007-9076-0.
In this paper, we present a novel and fully integrated centrifugal microfluidic "lab-on-a-disk" for rapid colorimetric assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t=150 s in passive, globally hydrophilized structures which obviate the need for intricate local hydrophobic surface patterning. Our technology features a plasma extraction structure (V=500 nL, CV<5%) where the purified plasma (cRBC<0.11%) is centrifugally separated, metered by an overflow and subsequently extracted by a siphon-based principle through a hydrophilic extraction channel into the detection chamber.
在本文中，我们展示了一种新颖的全集成离心式微流控“芯片实验室”，用于在人全血中进行快速比色测定。包括血液采样、计量、血浆提取和最终光学检测在内的所有关键步骤，均在被动式、整体亲水化结构中于t = 150秒内完成，无需复杂的局部疏水表面图案化。我们的技术具有一个血浆提取结构（V = 500 nL，CV < 5%），在该结构中，纯化后的血浆（cRBC < 0.11%）通过离心分离，经溢流计量，随后基于虹吸原理通过亲水性提取通道提取到检测室中。
