胃缺血时的氧化应激和缺氧诱导因子 1α 表达。
Oxidative stress and hypoxia-induced factor 1α expression in gastric ischemia.
机构信息
Department of Anesthesiology, The First Hospital of Lanzhou University, Lanzhou 73000, Gansu Province, China.
出版信息
World J Gastroenterol. 2011 Apr 14;17(14):1915-22. doi: 10.3748/wjg.v17.i14.1915.
AIM
To investigate the relation of reactive oxygen species (ROS) to hypoxia induced factor 1α (HIF-1α) in gastric ischemia.
METHODS
The animal model of gastric ischemia reperfusion was established by placing an elastic rubber band on the proximal part of the bilateral lower limb for ligature for 3 h and reperfusion for 0, 1, 3, 6, 12 or 24 h. Ischemic post-conditioning, three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were conducted before reperfusion. Histological and immunohistochemical methods were used to assess the gastric oxidative damage and the expression of HIF1-α in gastric ischemia. The malondialdehyde (MDA) content and superoxide dismutase (SOD), xanthine oxidase (XOD) and myeloperoxidase (MPO) activities were determined by colorimetric assays.
RESULTS
Ischemic post-conditioning can reduce post-ischemic oxidative stress and the expression of HIF-1α of gastric tissue resulting from limb ischemia reperfusion injury. MDA, SOD, XOD and MPO were regarded as indexes for mucosal injuries from ROS, and ROS was found to affect the expression of HIF-1α under gastric ischemic conditions.
CONCLUSION
ROS affects HIF-1α expression under gastric ischemic conditions induced by limb ischemia reperfusion injury. Therefore, ROS can regulate HIF-1α expression in gastric ischemia.
目的
探讨活性氧（ROS）与胃缺血缺氧诱导因子 1α（HIF-1α）的关系。
方法
通过在双侧下肢近端放置弹性橡皮筋结扎 3 h 并再灌注 0、1、3、6、12 或 24 h 来建立胃缺血再灌注动物模型。在再灌注前进行缺血后处理，即 3 个循环的 30 s 再灌注和 30 s 股动脉再闭塞。采用组织学和免疫组织化学方法评估胃氧化损伤和胃缺血中 HIF1-α的表达。通过比色法测定丙二醛（MDA）含量和超氧化物歧化酶（SOD）、黄嘌呤氧化酶（XOD）和髓过氧化物酶（MPO）活性。
结果
肢体缺血再灌注损伤后，缺血后处理可减轻缺血后氧化应激和胃组织 HIF-1α的表达。MDA、SOD、XOD 和 MPO 被视为 ROS 引起的黏膜损伤指标，并且在胃缺血条件下 ROS 被发现影响 HIF-1α的表达。
结论
ROS 影响肢体缺血再灌注损伤诱导的胃缺血条件下 HIF-1α的表达。因此，ROS 可以调节胃缺血时 HIF-1α的表达。
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